A spontaneous mutant of microcystin biosynthesis: genetic characterization and effect on Daphnia
| Autor: | Brett A. Neilan, Melanie Kaebernick, Kirsten Christoffersen, Thomas Rohrlack |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cyanobacteria
Microcystins Bacterial Toxins Microcystin Peptides Cyclic Microbiology Daphnia Bacterial Proteins Microcystis polycyclic compounds Animals Humans Microcystis aeruginosa Enzyme Inhibitors Peptide Synthases Chromatography High Pressure Liquid reproductive and urinary physiology Ecology Evolution Behavior and Systematics chemistry.chemical_classification biology fungi Wild type biology.organism_classification Pulicaria Diet Survival Rate chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Daphnia galeata |
| Zdroj: | Environmental Microbiology. 3:669-679 |
| ISSN: | 1462-2920 1462-2912 |
| DOI: | 10.1046/j.1462-2920.2001.00241.x |
| Popis: | Microcystis aeruginosa strain MRC is unique in its' possession of the mcyA-J gene cluster, which encodes microcystin synthetase, but its' inability to produce microcystins. M. aeruginosa strain MRD is genetically identical to MRC at numerous genomic loci examined, but produces a variety of microcystins, mainly with the amino acid tyrosine in the molecule. Zooplankton studies with Daphnia galeata and D. pulicaria, using the mutant (MRC) and its' wild type (MRD), showed for the first time that microcystins other than microcystin-LR can be responsible for the poisoning of Daphnia by Microcystis. Regardless of microcystin content, both Daphnia exhibited significantly reduced ingestion rates when fed with either strain of M. aeruginosa compared with the green alga Scenedesmus acutus. A disruption of the molting process in both Daphnia spp. was noted when these species were fed with MRC cells. Such symptoms on Daphnia have not been previously reported for cyanobacteria and may point to a bioactive compound, other than microcystin, which inhibits the hardening of protein-chitin complexes in Daphnia. |
| Databáze: | OpenAIRE |
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