Trimethylamine-N-oxide is present in human follicular fluid and is a negative predictor of embryo quality
| Autor: | F Liu, Uwe J. F. Tietge, Annemieke Hoek, Ruxandra A. Nagy, Josephine L. C. Anderson, Congzhuo Jia, I. Homminga |
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| Přispěvatelé: | Reproductive Origins of Adult Health and Disease (ROAHD), Lifestyle Medicine (LM), Center for Liver, Digestive and Metabolic Diseases (CLDM) |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Metabolite
Trimethylamine N-oxide TMAO Fertilization in Vitro METABOLISM liver OOCYTE Andrology 03 medical and health sciences chemistry.chemical_compound Methylamines 0302 clinical medicine Human fertilization Pregnancy choline Follicular phase medicine microbiota IMPROVES Choline Humans Carnitine intestine 030304 developmental biology Netherlands fertility 0303 health sciences 030219 obstetrics & reproductive medicine business.industry Rehabilitation Obstetrics and Gynecology WOMEN Oxides Follicular fluid l-carnitine Follicular Fluid BODY-MASS INDEX Cross-Sectional Studies MICROBIOME Reproductive Medicine chemistry OBESITY gamma-butyrobetaine Female business Embryo quality medicine.drug |
| Zdroj: | Human Reproduction, 35(1), 81-88. Oxford University Press |
| ISSN: | 0268-1161 |
| Popis: | STUDY QUESTION Are levels of trimethylamine-N-oxide (TMAO) in human follicular fluid (FF) related to IVF outcomes? SUMMARY ANSWER Higher levels of TMAO are a negative predictor of oocyte fertilization and embryo quality. WHAT IS KNOWN ALREADY TMAO is a metabolic product of dietary choline and l-carnitine produced via subsequent enzymatic modifications by the intestinal microbiota and hepatocytes. TMAO promotes inflammatory and oxidative stress pathways and has been characterized as a causative biomarker for the development of cardiometabolic disease. STUDY DESIGN, SIZE, DURATION For the present cross-sectional study, samples (FF and plasma) from 431 modified natural cycle (MNC)-IVF cycles of 132 patients were collected prospectively between October 2014 and March 2018 in a single academic medical center. PARTICIPANTS/MATERIALS, SETTING, METHODS TMAO and its precursors (choline, l-carnitine and gamma-butyrobetaine) were measured by ultra-high-performance liquid chromatography/mass spectrometry in (i) matched FF and plasma from 63 MNC-IVF cycles, in order to compare metabolite levels in the two matrices and (ii) FF from 232 MNC-IVF cycles in which only one oocyte was retrieved at follicular puncture. The association between metabolite levels and oocyte fertilization, embryo fragmentation percentage, embryo quality and the occurrence of pregnancy was analyzed using multilevel generalized estimating equations with adjustment for patient and cycle characteristics. MAIN RESULTS AND THE ROLE OF CHANCE The level of choline was higher in FF as compared to matched plasma (P LIMITATIONS, REASONS FOR CAUTION The individual contributions of diet, gut bacteria and liver to the metabolite pools have not been quantified in this analysis. WIDER IMPLICATIONS OF THE FINDINGS More research on the contribution of diet and the effect of gut bacteria on FF TMAO is warranted. Since TMAO integrates diet, microbiota and genetic setup of the person, our results indicate potential important clinical implications for its use as biomarker for lifestyle interventions to improve fertility. STUDY FUNDING/COMPETING INTEREST(S) No external funding was received for this project. The Department of Obstetrics and Gynecology of the University Medical Center Groningen received an unrestricted educational grant of Ferring Pharmaceutical BV, the Netherlands. The authors have no other conflicts of interest. TRIAL REGISTRATION NUMBER Netherlands Trial Register number NTR4409. |
| Databáze: | OpenAIRE |
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