Replication and polypeptide synthesis of Mill Door/79, an orbivirus isolated from ticks from a seabird colony in Scotland
| Autor: | Norman F. Moore, S. M. Eley, J.S.K. Pullin, R. P. Spence, Patricia A. Nuttall |
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| Rok vydání: | 1985 |
| Předmět: |
Genes
Viral Proteolysis viruses Immunology Reoviridae Virus Replication Microbiology Virus Cell Line Birds Viral Proteins Ticks Virology Chlorocebus aethiops medicine Animals Gene RNA Double-Stranded Orbivirus medicine.diagnostic_test biology RNA biology.organism_classification Viral replication Scotland Insect Science Vero cell RNA Viral Research Article |
| Zdroj: | Journal of virology. 53(2) |
| ISSN: | 0022-538X |
| Popis: | The replication and polypeptide synthesis of orbivirus isolate Mill Door/79, a member of the Kemerovo serogroup, were studied. In Vero cells cell-associated virus exceeded cell-free virus by about 2 log10 PFU/ml. Attempts to purify the virus resulted in the demonstration of five polypeptides. Thirteen virus-induced polypeptides and 10 segments of double-stranded RNA were identified in infected cells. Partial proteolysis demonstrated homology between some polypeptides. |
| Databáze: | OpenAIRE |
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