Impulsive pressurization of neuronal cells for traumatic brain injury study
| Autor: | Jeong Soon Lee, Matthew Nienaber, Ruqiang Feng, Jung Yul Lim |
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| Rok vydání: | 2011 |
| Předmět: |
Issue 56
Traumatic Brain Injury Neurite Traumatic brain injury General Chemical Engineering Cytological Techniques Bioengineering General Biochemistry Genetics and Molecular Biology Neuroblastoma Cabin pressurization Blast Injuries Neuronal Cells Cell Line Tumor medicine Pressure Humans Impulsive Pressurization Neurons General Immunology and Microbiology Chemistry General Neuroscience medicine.disease Biomechanical Phenomena Blast-TBI Brain Injuries Biophysics Neuroscience |
| Zdroj: | Journal of Visualized Experiments : JoVE |
| ISSN: | 1940-087X |
| Popis: | A novel impulsive cell pressurization experiment has been developed using a Kolsky bar device to investigate blast-induced traumatic brain injury (TBI). We demonstrate in this video article how blast TBI-relevant impulsive pressurization is applied to the neuronal cells in vitro. This is achieved by using well-controlled pressure pulse created by a specialized Kolsky bar device, with complete pressure history within the cell pressurization chamber recorded. Pressurized neuronal cells are inspected immediately after pressurization, or further incubated to examine the long-term effects of impulsive pressurization on neurite/axonal outgrowth, neuronal gene expression, apoptosis, etc. We observed that impulsive pressurization at about 2 MPa induces distinct neurite loss relative to unpressurized cells. Our technique provides a novel method to investigate the molecular/cellular mechanisms of blast TBI, via impulsive pressurization of brain cells at well-controlled pressure magnitude and duration. |
| Databáze: | OpenAIRE |
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