Dietary mung bean protein reduces high-fat diet-induced weight gain by modulating host bile acid metabolism in a gut microbiota-dependent manner
| Autor: | Asuka Sutou, Mitsutaka Kohno, Ikuo Kimura, Keita Watanabe, Hitoshi Watanabe, Xuan Li, Takayasu Motoyama, Miki Igarashi, Junki Miyamoto, Nobuhiko Tachibana, Akiho Nakatani, Hiroshi Inoue |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male medicine.medical_specialty Firmicutes medicine.drug_class medicine.medical_treatment Biophysics Adipose tissue Gut flora Diet High-Fat Weight Gain Biochemistry Bile Acids and Salts 03 medical and health sciences Feces Internal medicine medicine Animals Germ-Free Life Molecular Biology Cecum Plant Proteins 030109 nutrition & dietetics biology Bile acid Chemistry Insulin Vigna digestive oral and skin physiology Cell Biology biology.organism_classification medicine.disease Gastrointestinal Microbiome Mice Inbred C57BL 030104 developmental biology Endocrinology Phenotype Dietary Proteins medicine.symptom Steatosis Weight gain |
| Zdroj: | Biochemical and biophysical research communications. 501(4) |
| ISSN: | 1090-2104 |
| Popis: | The 8-globulin-rich mung bean protein (MPI) suppresses hepatic lipogenesis in rodent models and reduces fasting plasma glucose and insulin levels in obese adults. However, its effects on mitigating high fat diet (HFD)-induced obesity and the mechanism underlying these effects remain to be elucidated. Herein, we examined the metabolic phenotype, intestinal bile acid (BA) pool, and gut microbiota of conventionally raised (CONV-R) male C57BL/6 mice and germ-free (GF) mice that were randomized to receive either regular HFD or HFD containing mung bean protein isolate (MPI) instead of the dairy protein present in regular HFD. MPI intake significantly reduced HFD-induced weight gain and adipose tissue accumulation, and attenuated hepatic steatosis. Enhancement in the secretion of intestinal glucagon-like peptide-1 (GLP-1) and an enlarged cecal and fecal BA pool of dramatically elevated secondary/primary BA ratio were observed in mice that had consumed MPI. These effects were abolished in GF mice, indicating that the effects were dependent upon the presence of the microbiota. As revealed by 16S rRNA gene sequence analysis, MPI intake also elicited dramatic changes in the gut microbiome, such as an expansion of taxa belonging to the phylum Bacteroidetes along with a reduced abundance of the Firmicutes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect