Sphingomonas hengshuiensis sp. nov., isolated from lake wetland
| Autor: | Guo Jiping, Zhang Zhiqiang, Hongliang Liu, Tingting Wang, Kuijing Liang, Shuzhen Wei, Caifeng Zhang, Qian Wang |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
DNA
Bacterial China Spermidine Ubiquinone Molecular Sequence Data medicine.disease_cause Sphingomonas Microbiology Glycosphingolipids Phylogenetics RNA Ribosomal 16S Botany medicine Phospholipids Phylogeny Soil Microbiology Ecology Evolution Behavior and Systematics Base Composition biology Strain (chemistry) Fatty Acids Sequence Analysis DNA General Medicine biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques Lakes genomic DNA Wetlands Soil microbiology Sphingomonas asaccharolytica Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 65:4644-4649 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A polyphasic taxonomic study was undertaken to establish the status of a novel bacterium, designated strain WHSC-8T, which was isolated from soil of Hengshui Lake Wetland Reserve in Hebei province, northern China. Colonies of this strain were yellow and cells were rod-shaped, polar-flagellated and obligately aerobic, exhibiting negative Gram reaction. The strain was able to grow at 0–1 % (w/v) NaCl, pH 5–10 and 20–35 °C, with optimal growth occurring at pH 7.0 and 28 °C without NaCl. Chemotaxonomic data revealed that strain WHSC-8T possesses ubiquinone Q-10 as the predominant respiratory quinone, C18 : 1ω7c, C16 : 0 and summed feature 3 (C16 : 1ω7c and/or iso-C15 : 0 2-OH) as the major fatty acids, and sym-homospermidine as the major polyamine. Sphingomonadaceae-specific sphingoglycolipid was detected in the polar lipid patterns. The G+C content of the genomic DNA was 68.7 mol%. All of the above characters corroborated the assignment of the novel strain to the genus Sphingomonas. Strain WHSC-8T shared less than 97.0 % 16S rRNA gene sequence similarity with the type strains of other species of the genus Sphingomonas, except for Sphingomonas asaccharolytica DSM 10564T (97.5 %). The low DNA–DNA relatedness value and distinct phenotypic and chemotaxonomic characteristics distinguished strain WHSC-8T from closely related species of the genus Sphingomonas. Therefore, strain WHSC-8T represents a novel species of the genus Sphingomonas, for which the name Sphingomonas hengshuiensis sp. nov. is proposed. The type strain is WHSC-8T ( = KCTC 42455T = CCTCC AB 2015265T). |
| Databáze: | OpenAIRE |
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