Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation
| Autor: | Blondine Agus, Federica Deidda, Cristina Porcu, Cinzia Lobina, Elisabetta Coluccia, Riccardo Melis, Susanna Salvadori |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0106 biological sciences 0301 basic medicine ribosomal genes comparative cytogenetics lcsh:QH426-470 Color DNA Ribosomal 010603 evolutionary biology 01 natural sciences Chromosomes Fluorescence Article 03 medical and health sciences Species Specificity FISH Indian Ocean Islands Muraena Genetics Gymnothorax Animals Gene family Muraenidae Indian Ocean In Situ Hybridization Fluorescence Phylogeny Genetics (clinical) Synteny Eels biology Phylogenetic tree RNA Ribosomal 5S Chromosome Mapping Chromosome Karyotype Ribosomal RNA biology.organism_classification lcsh:Genetics 030104 developmental biology Evolutionary biology Karyotyping Cytogenetic Analysis Female |
| Zdroj: | Genes, Vol 11, Iss 1319, p 1319 (2020) Genes Volume 11 Issue 11 |
| ISSN: | 2073-4425 |
| Popis: | The Muraenidae is one of the largest and most complex anguilliform families. Despite their abundance and important ecological roles, morays are little studied, especially cytogenetically, and both their phylogenetic relationships and the taxonomy of their genera are controversial. With the aim of extending the karyology of this fish group, the chromosomal mapping of the 5S ribosomal gene family was performed on seven species belonging to the genera Muraena and Gymnothorax from both the Atlantic and Pacific oceans. Fluorescence in situ hybridisation (FISH) experiments were realized using species-specific 5S rDNA probes in addition, two-colour FISH was performed to investigate the possible association with the 45S ribosomal gene family. Multiple 5S rDNA clusters, located either in species-specific or in possibly homoeologous chromosomes, were found. Either a syntenic or different chromosomal location of the two ribosomal genes was detected. Our results revealed variability in the number and location of 5S rDNA clusters and confirmed a substantial conservation of the number and location of the 45S rDNA. |
| Databáze: | OpenAIRE |
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