CC and C chemokine expression in pulmonary sarcoidosis
Autor: | Vítězslav Kolek, Martin Petrek, R M du Bois, Jaroslava Szotkowska |
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Rok vydání: | 2002 |
Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Systemic disease Chemokine Gene Expression Enzyme-Linked Immunosorbent Assay CCL5 Sarcoidosis Pulmonary Gene expression medicine Humans RNA Messenger Chemokine CCL4 Chemokine CCL5 Chemokine CCL2 Chemokine CCL3 Messenger RNA medicine.diagnostic_test biology Reverse Transcriptase Polymerase Chain Reaction business.industry Monocyte Macrophage Inflammatory Proteins medicine.disease Chemokines C Bronchoalveolar lavage medicine.anatomical_structure Chemokines CC Immunology biology.protein Female Sarcoidosis business Bronchoalveolar Lavage Fluid |
Zdroj: | European Respiratory Journal. 20:1206-1212 |
ISSN: | 1399-3003 0903-1936 |
Popis: | The chemokines RANTES (regulated on activation, T-cell expressed and secreted; CC chemokine ligand (CCL)-5) and monocyte inflammatory protein (MIP)-1alpha (CCL-3) have been implicated in the development of alveolitis in pulmonary sarcoidosis. The novel C chemokine single cysteine motif (SCM)-1alpha (XCL-1) and the CC chemokine monocyte chemoattractant protein (MCP)-1 (CCL-2) are also mononuclear-cell attractants and represent alternative candidate mediators of alveolitis. Therefore, the expression of MCP-1 and SCM-1alpha was investigated together with the expression of RANTES and MIP-1alpha in bronchoalveolar lavage fluid (BALF) from control subjects and patients with sarcoidosis. The relationship between chemokine expression and sarcoidosis clinical course was also explored. Messenger ribonucleic acid (mRNA) expression for all four chemokines was determined by semiquantitative reverse transcriptase-polymerase chain reaction of RNA extracted from unseparated bronchoalveolar cells (17 patients, 12 controls). RANTES, MIP-1alpha and MCP-1 proteins were measured by enzyme-linked immunosorbent assay of unconcentrated BALF (60 patients, 17 controls). MCP-1, and namely RANTES and SCM-1alpha mRNA expression was upregulated in sarcoidosis, particularly in patients with more advanced disease. RANTES, and namely MCP-1 concentrations were elevated in BALF samples obtained from patients; MCP-1 levels were most increased in patients with chest radiographic stage 2 disease and also in patients with persistent and recurrent disease. In conclusion, chemokines monocyte chemotactive protein-1 and single cysteine motif-1alpha are, in addition to RANTES, associated with the development of alveolitis in sarcoidosis and their expression parallels the disease course. |
Databáze: | OpenAIRE |
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