CREB deletion increases resilience to stress and downregulates inflammatory gene expression in the hippocampus
| Autor: | Melissa T. Manners, Darrell Eacret, Xin Liu, Max Schechter, Julie A. Blendy, Julia K. Brynildsen |
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| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Immunology Gene Expression Hippocampus Inflammation Biology CREB Article Mice 03 medical and health sciences Behavioral Neuroscience 0302 clinical medicine Gene expression medicine Animals Phosphorylation Cyclic AMP Response Element-Binding Protein Gene Transcription factor Mice Knockout Depression Endocrine and Autonomic Systems Wild type Brain Toll-Like Receptor 1 Antidepressive Agents Temporal Lobe Cell biology Mice Inbred C57BL 030104 developmental biology Gene Expression Regulation biology.protein Cyclic AMP Response Element medicine.symptom Stress Psychological 030217 neurology & neurosurgery |
| Zdroj: | Brain Behav Immun |
| ISSN: | 0889-1591 |
| Popis: | The transcription factor CREB (cyclic AMP response element (CRE)-binding protein) is implicated in the pathophysiology and treatment of depression. Structural and functional studies in both animals and humans suggest that abnormalities of the hippocampus may play a role in depression. CREB regulates thousands of genes, yet to date, only a handful that mediate depression or antidepressant response have been identified as relevant CREB targets. In order to comprehensively identify genes regulated by CREB in the hippocampus, we employed translating ribosome affinity purification (TRAP) to detect actively translating mRNAs in wild type and CREB-deficient mice. Using Creb(loxP/loxP); Rosa(LSL-GFP-L10a) mice, we conducted whole genome sequencing to identify transcripts only in cells that lack CREB, as introduction of Crerecombinase simultaneously deleted CREB and expressed GFP-tagged L10a ribosomes that enabled TRAP. We identified over 200 downregulated genes predominantly associated with inflammation and the immune system, including toll-like receptor 1 (TLR1). To determine if baseline disruption in gene expression in the hippocampus of CREB-deficient mice can modulate behavior, we used unpredictable chronic mild stress (UCMS) to produce a set of behavioral alterations with strong validity for depression. We found that CREB-deficient mice demonstrated resilience to the physiological effects of UCMS and also showed changes in affective behaviors specifically in the presence of stress. TLR1 expression was increased following UCMS in control but not in CREB-deficient mice. The results suggest that CREB-mediated regulation of immune system and inflammatory factors may provide additional targets for the treatment of depression. |
| Databáze: | OpenAIRE |
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