Extract of Pelargonium sidoides (EPs® 7630) inhibits the interactions of group A-streptococci and host epithelia in vitro
| Autor: | Irina Jung, Uwe Frank, Andreas Conrad, Felipe Carrapatoso, Diane Tioua, F. Daschner, Inge Engels, Caroline Lallemand |
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| Rok vydání: | 2007 |
| Předmět: |
Streptococcus pyogenes
medicine.drug_class Cell Antibiotics Pharmaceutical Science Microbial Sensitivity Tests Pelargonium sidoides Pelargonium Biology Plant Roots Bacterial Adhesion Microbiology Drug Discovery medicine Fluorescence microscope Humans Respiratory Tract Infections Cells Cultured Pharmacology Dose-Response Relationship Drug Plant Extracts Intracellular parasite Epithelial Cells Adhesion biology.organism_classification In vitro Anti-Bacterial Agents medicine.anatomical_structure Complementary and alternative medicine Molecular Medicine Bacteria Phytotherapy |
| Zdroj: | Phytomedicine. 14:52-59 |
| ISSN: | 0944-7113 |
| Popis: | EPs 7630 is an extract of the root of the South African geranium Pelargonium sidoides. Clinical data have shown that this herbal drug preparation can be used to treat upper respiratory tract infections (URTI). The objective of this study was to investigate the impact of EPs 7630 on group A-streptococci (GAS) adhering to and invading host epithelial cells in vitro. Adhesion was assessed by a flow cytometric adhesion assay using calcein-AM-stained S. pyogenes (DSM 2071) as test organism, and HEp-2 cells and buccal epithelial cells (BEC) as substrata. For cell invasion, HEp-2 cell monolayers were infected with S. pyogenes. Intracellular bacteria were determined using a penicillin/gentamicin-protection assay. EPs 7630 was applied in therapeutically relevant concentrations between 0 and 30 microg/ml in both test systems. Compared to controls, EPs 7630 significantly reduced GAS adhesion to HEp-2 cells in a concentration-dependent manner by up to 46% (p |
| Databáze: | OpenAIRE |
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