Identification and Quantification of Quercetin, A Major Constituent of Artocarpus altilis by Targeting Related Genes of Apoptosis and Cell Cycle: In Vitro Cytotoxic Activity Against Human Lung Carcinoma Cell Lines
| Autor: | Mohammad Syaiful Bahari Abdull Rasad, Tara Jalal, Al'aina Yuhainis Firus Khan, Mardhiah Mohammad, Hatim A. Natto, Muhammad Nor Omar, Ridhwan Abdul Wahab, Muhammad Farid Johan, Mohd Arifin Kaderi |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Cancer Research Lung Neoplasms Cell Medicine (miscellaneous) Apoptosis In Vitro Techniques Antioxidants 03 medical and health sciences Artocarpus 0302 clinical medicine food Cell Line Tumor medicine Humans 030109 nutrition & dietetics Nutrition and Dietetics biology Chemistry Plant Extracts Cytochrome c Artocarpus altilis Cell Cycle Cell cycle biology.organism_classification Molecular biology food.food medicine.anatomical_structure Oncology Cell culture 030220 oncology & carcinogenesis biology.protein Pulp (tooth) Quercetin |
| Zdroj: | Nutrition and cancer. 71(5) |
| ISSN: | 1532-7914 |
| Popis: | Nine phenolic compounds were identified and quantified in Artocarpus altilia fruit. One of the main compounds was quercetin, which is the major class of flavonoids has been identified and quantified in pulp part of A. altilis fruit of methanol extract. The aim of this study was to evaluate in vitro cytotoxic assay. Inhibitory concentration 50% concentration was determined using trypan blue exclusion assay. Apoptosis induction and cell cycle regulation were studied by flow cytometric analysis. The expression of apoptosis and cell cycle-related regulatory genes were assessed by RT-qPCR study of the methanol extract of pulp part on human lung carcinoma (A549) cell line. A significant increase of cells at G2/M phases was detected (P |
| Databáze: | OpenAIRE |
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