Evaluation of in vitro enzymatic and non-enzymatic antioxidant properites of leaf extract from Alpinia Purpurata (Vieill.) K. Schum
| Autor: | Thangarajan Starlin, Chinthamony Arul Raj, Dominic Sophia, Velliyur Kanniappan Gopalakrishnan, Muthian Ahalliya Rathi, Paramasivam Ragavendran |
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| Rok vydání: | 2011 |
| Předmět: |
0301 basic medicine
Antioxidant medicine.medical_treatment Antioxidants Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound Alpinia purpurata medicine Pharmacology (medical) Food science chemistry.chemical_classification 030102 biochemistry & molecular biology Vitamin C biology business.industry Plant Extracts Glutathione peroxidase General Medicine Glutathione biology.organism_classification Biotechnology Enzymes Plant Leaves Complementary and alternative medicine chemistry Catalase biology.protein Alpinia Lipid Peroxidation business Catechol Oxidase Peroxidase |
| Zdroj: | Chinese journal of integrative medicine. 22(9) |
| ISSN: | 1672-0415 |
| Popis: | To evaluate the enzymatic and non-enzymatic antioxidants of leaf extract from Alpinia purpurata. One gram of fresh leaf of Alpinia purpurata was grinded in 2 mL of 50% ethanol and centrifuged at 10,000×g at 4°C for 10 min. The supernatant obtained was used within 4 h for various enzymatic antioxidants assays like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione S-transferase (GST), ascorbate oxidase, peroxidase, polyphenol oxidase (PPO) and non-enzymatic antioxidants such as vitamin C, total reduced glutathione (TRG) and lipid peroxidation (LPO). The leaf extract of Alpinia purpurata possess antioxidants like vitamin C 472.92±6.80 μg/mg protein, GST 372.11±5.70 μmol of 1-chloro 2,4 dinitrobenzene (CDNB)-reduced glutathione (GSH) conjugate formed/min/mg protein, GPx 281.69±6.43 μg of glutathione oxidized/min/mg protein, peroxidases 173.12±9.40 μmol/g tissue, TRG 75.27±3.55 μg/mg protein, SOD 58.03±2.11 U/mg protein, CAT 46.70±2.35 μmol of H2O2 consumed/min/mg protein in high amount whereas ascorbate oxidase 17.41±2.46 U/g tissue, LPO 2.71±0.14 nmol/L of malondialdehyde formed/min/mg protein and PPO 1.14±0.11 μmol/g tissue in moderate amount. Alpinia purpurata has the potential to scavenge the free radicals and protect against oxidative stress causing diseases. In future, Alpinia purpurata may serve as a good pharmacotherapeutic agent. |
| Databáze: | OpenAIRE |
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