| Autor: |
Xianjuan Hou, Shen, Zhen, Li, Na, Xiaowei Kong, Kangliang Sheng, Jingmin Wang, Wang, Yongzhong |
| Rok vydání: |
2020 |
| DOI: |
10.6084/m9.figshare.12096594.v1 |
| Popis: |
Additional file 1: Figure S1. Codon-optimized Aophytase gene (Genbank accession NO. MN688990) for Pichia pastoris expression. Note: GAATTC is EcoRI recognition site; GTCGAC is SalI recognition site. Figure S2. Linearized pPICZαA-Aophytase by SacI digestion. Plasmid pPICZαA-Aophytase was digested by SacI, and detected by DNA agarose gel electrophoresis. (M) DL5000 DNA Marker; (1) plasmid pPICZαA-Aophytase; (2) Linearized pPICZαA-Aophytase shows a single band with its length more than 5000 bp in the agarose gel, well agreeing with its length of 5767 bp. Figure S3. The standard curve for Pi determination. One mL of AMES solution was added to 1ml phosphorus standard solution followed by 50 ºC water-bath for 20 min. The OD values of the reaction mixtures were determined at λ700 nm. The standard curve of OD700 v.s. phosphorus concentration was plotted. Through linear fitting and regression, the linear equation is y = 6.2417x + 0.0723 and R2 = 0.9971. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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