Mitochondrial genomes reveal recombination in the presumed asexual Fusarium oxysporum species complex
| Autor: | Cees Waalwijk, Theo van der Lee, Balรกzs Brankovics, G. Sybren de Hoog, Martijn Rep, Anne D. van Diepeningen, Peter van Dam |
|---|---|
| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Medical Mycology, Evolutionary and Population Biology (IBED, FNWI), Molecular Plant Pathology (SILS, FNWI) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Mitochondrial DNA lcsh:QH426-470 lcsh:Biotechnology Population Biology 01 natural sciences Genome Mitochondrial recombination DNA sequencing 03 medical and health sciences Biointeractions and Plant Health Fusarium Mitochondrial genome Phylogenomics lcsh:TP248.13-248.65 Genetics Clade education Conserved Sequence Phylogeny Comparative genomics Recombination Genetic education.field_of_study Phylogenetic tree Entomology & Disease Management Genetic Variation Genomics Introns lcsh:Genetics 030104 developmental biology Evolutionary biology Genome Mitochondrial EPS 010606 plant biology & botany Biotechnology Research Article |
| Zdroj: | BMC Genomics 18 (2017) 1 BMC Genomics, 18(1):735. BioMed Central BMC Genomics BMC Genomics, Vol 18, Iss 1, Pp 1-14 (2017) BMC Genomics, 18:735. BioMed Central BMC Genomics, 18(1) |
| ISSN: | 1471-2164 |
| Popis: | Background The Fusarium oxysporum species complex (FOSC) contains several phylogenetic lineages. Phylogenetic studies identified two to three major clades within the FOSC. The mitochondrial sequences are highly informative phylogenetic markers, but have been mostly neglected due to technical difficulties. Results A total of 61 complete mitogenomes of FOSC strains were de novo assembled and annotated. Length variations and intron patterns support the separation of three phylogenetic species. The variable region of the mitogenome that is typical for the genus Fusarium shows two new variants in the FOSC. The variant typical for Fusarium is found in members of all three clades, while variant 2 is found in clades 2 and 3 and variant 3 only in clade 2. The extended set of loci analyzed using a new implementation of the genealogical concordance species recognition method support the identification of three phylogenetic species within the FOSC. Comparative analysis of the mitogenomes in the FOSC revealed ongoing mitochondrial recombination within, but not between phylogenetic species. Conclusions The recombination indicates the presence of a parasexual cycle in F. oxysporum. The obstacles hindering the usage of the mitogenomes are resolved by using next generation sequencing and selective genome assemblers, such as GRAbB. Complete mitogenome sequences offer a stable basis and reference point for phylogenetic and population genetic studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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