Expression of multilectin receptors and comparative FITC–dextran uptake by human dendritic cells
| Autor: | Derek N.J. Hart, Slavica Vuckovic, Masato Kato, Alexander D. McLellan, Teresa K. Neil, D.B. Fearnley |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
medicine.medical_treatment
Immunology Mannose Receptors Cell Surface Biology Monocytes Minor Histocompatibility Antigens chemistry.chemical_compound Antigen Antigens CD Lectins Tumor Cells Cultured medicine Animals Humans Immunology and Allergy Lectins C-Type RNA Messenger Receptor Fluorescent Dyes Messenger RNA Membrane Glycoproteins Macrophages Dextrans Dendritic Cells General Medicine Immunotherapy Isoquinolines Hodgkin Disease Molecular biology Cell biology Receptors Antigen Mannose-Binding Lectins chemistry Langerhans Cells COS Cells Pinocytosis FITC-dextran Fluorescein-5-isothiocyanate Mannose Receptor Function (biology) Intracellular |
| Zdroj: | International Immunology. 12:1511-1519 |
| ISSN: | 1460-2377 0953-8178 |
| DOI: | 10.1093/intimm/12.11.1511 |
| Popis: | Dendritic cells (DC) are potent antigen-presenting cells and understanding their mechanisms of antigen uptake is important for loading DC with antigen for immunotherapy. The multilectin receptors, DEC-205 and macrophage mannose receptor (MMR), are potential antigen-uptake receptors; therefore, we examined their expression and FITC-dextran uptake by various human DC preparations. The RT-PCR analysis detected low levels of DEC-205 mRNA in immature blood DC, Langerhans cells (LC) and immature monocyte-derived DC (Mo-DC). Its mRNA expression increased markedly upon activation, indicating that DEC-205 is an activation-associated molecule. In Mo-DC, the expression of cell-surface DEC-205 increased markedly during maturation. In blood DC, however, the cell-surface expression of DEC-205 did not change during activation, suggesting the presence of a large intracellular pool of DEC-205 or post-transcriptional regulation. Immature Mo-DC expressed abundant MMR, but its expression diminished upon maturation. Blood DC and LC did not express detectable levels of the MMR. FITC-dextran uptake by both immature and activated blood DC was 30- to 70-fold less than that of LC, immature Mo-DC and macrophages. In contrast to immature Mo-DC, the FITC-dextran uptake by LC was not inhibited effectively by mannose, an inhibitor for MMR-mediated FITC-dextran uptake. Thus, unlike Mo-DC, blood DC and LC do not use the MMR for carbohydrate-conjugated antigen uptake and alternative receptors may yet be defined on these DC. Therefore, DEC-205 may have a different specificity as an antigen uptake receptor or contribute to an alternative DC function. |
| Databáze: | OpenAIRE |
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