Human blood MAIT cell subsets defined using MR1 tetramers

Autor: Michael N. T. Souter, Yves d'Udekem, Igor E. Konstantinov, James McCluskey, Sidonia B G Eckle, Torsten Seemann, Timothy P. Stinear, Hui-Fern Koay, Paul J Neeson, Dale I. Godfrey, Kirstie M. Mangas, Nicholas A Gherardin, Adam P Uldrich, Stuart P. Berzins, David P. Fairlie, Daniel G. Pellicci, David Ritchie
Rok vydání: 2018
Předmět:
0301 basic medicine
Aging
Immunology
Population
Receptors
Antigen
T-Cell
Receptor specificity
T-Cell Antigen Receptor Specificity
Cell Separation
Mucosal associated invariant T cell
Biology
Lymphocyte Activation
Mucosal-Associated Invariant T Cells
Minor Histocompatibility Antigens
03 medical and health sciences
0302 clinical medicine
T-Lymphocyte Subsets
unconventional T cell
Humans
Immunology and Allergy
education
Cells
Cultured
education.field_of_study
Blood Cells
Human blood
Histocompatibility Antigens Class I
MR1
MAIT Cells
T cell
Cell Differentiation
Receptors
Antigen
T-Cell
gamma-delta
Original Articles
Cell Biology
Flow Cytometry
030104 developmental biology
T cell subset
Cytokines
Natural Killer T-Cells
Original Article
Tumor necrosis factor alpha
Human immunology
MAIT
Biomarkers
030215 immunology
Zdroj: Immunology and Cell Biology
ISSN: 1440-1711
0818-9641
Popis: Mucosal‐associated invariant T (MAIT) cells represent up to 10% of circulating human T cells. They are usually defined using combinations of non‐lineage‐specific (surrogate) markers such as anti‐TRAV1‐2, CD161, IL‐18Rα and CD26. The development of MR1‐Ag tetramers now permits the specific identification of MAIT cells based on T‐cell receptor specificity. Here, we compare these approaches for identifying MAIT cells and show that surrogate markers are not always accurate in identifying these cells, particularly the CD4+ fraction. Moreover, while all MAIT cell subsets produced comparable levels of IFNγ, TNF and IL‐17A, the CD4+ population produced more IL‐2 than the other subsets. In a human ontogeny study, we show that the frequencies of most MR1 tetramer+ MAIT cells, with the exception of CD4+ MAIT cells, increased from birth to about 25 years of age and declined thereafter. We also demonstrate a positive association between the frequency of MAIT cells and other unconventional T cells including Natural Killer T (NKT) cells and Vδ2+ γδ T cells. Accordingly, this study demonstrates that MAIT cells are phenotypically and functionally diverse, that surrogate markers may not reliably identify all of these cells, and that their numbers are regulated in an age‐dependent manner and correlate with NKT and Vδ2+ γδ T cells.
Databáze: OpenAIRE
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