NMR microsystem for label-free characterization of 3D nanoliter microtissues
| Autor: | Juergen Brugger, Gaurasundar M. Conley, Lukas Egli, Kyle J. Rodriguez, Jan Lichtenberg, Wolfgang Moritz, Erika Riva, Giovanni Boero, Marco Grisi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Computer science lcsh:Medicine Nanotechnology 02 engineering and technology Article 030218 nuclear medicine & medical imaging 03 medical and health sciences 0302 clinical medicine Non-alcoholic Fatty Liver Disease Microsystem Organoid Fatty acids lcsh:Science Spectroscopy 030304 developmental biology Label free 0303 health sciences Multidisciplinary Human liver in-vivo NMR Assay systems Scale (chemistry) lcsh:R 021001 nanoscience & nanotechnology Lipid Metabolism Biocompatible material NMR Electrical and electronic engineering 3. Good health Characterization (materials science) 030104 developmental biology Drug development Cell culture lcsh:Q Label-free 0210 nano-technology CMOS Technology Microfabrication |
| Zdroj: | Scientific Reports Scientific reports Scientific Reports, Vol 10, Iss 1, Pp 1-9 (2020) |
| DOI: | 10.1101/2020.04.26.062141 |
| Popis: | Performing chemical analysis at the nanoliter (nL) scale is of paramount importance for medicine, drug development, toxicology, and research. Despite the numerous methodologies available, a tool for obtaining chemical information non-invasively is still missing at this scale. Observer effects, sample destruction and complex preparatory procedures remain a necessary compromise1. Among non-invasive spectroscopic techniques, one able to provide holistic and highly resolved chemical information in-vivo is nuclear magnetic resonance (NMR)2,3. For its renowned informative power and ability to foster discoveries and life-saving applications4,5, efficient NMR at microscopic scales is highly sought after6–10, but so far technical limitations could not match the stringent necessities of microbiology, such as biocompatible handling, ease of use, and high throughput. Here we introduce a novel microsystem, which combines CMOS technology with 3D microfabrication, enabling nL NMR as a platform tool for non-invasive spectroscopy of organoids, 3D cell cultures, and early stage embryos. In this study we show its application to microlivers models simulating non-alcoholic fatty liver disease (NAFLD), demonstrating detection of lipid metabolism dynamics in a time frame of 14 days based on 117 measurements of single 3D human liver microtissues. |
| Databáze: | OpenAIRE |
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