Isolation and purification of a non-A, non-B hepatitis-associated microtubular aggregates protein
| Autor: | Yoshikazu Honda, Yoshiko Yoshiyama, Toshiro Maeda, Yohko K. Shimizu, Yasushi Ono, Ei Yamada, Toshio Shikata, Jun Kondo |
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| Rok vydání: | 1990 |
| Předmět: |
animal structures
Pan troglodytes medicine.drug_class Hepacivirus Molecular Sequence Data Buoyant density macromolecular substances Monoclonal antibody Microtubules Homology (biology) Antigen Microtubule Virology Centrifugation Density Gradient medicine Animals Amino Acid Sequence Antigens Cytoskeleton Peptide sequence Chromatography High Pressure Liquid biology biology.organism_classification Hepatitis C Peptide Fragments Molecular Weight Cytoskeletal Proteins Liver Biochemistry |
| Zdroj: | Journal of General Virology. 71:1999-2004 |
| ISSN: | 1465-2099 0022-1317 |
| Popis: | Blood-borne type non-A, non-B (NANB) hepatitis-associated microtubular aggregates protein was isolated and partially sequenced. The microtubular aggregates were isolated from the hepatocytes of NANB-infected chimpanzees and were found to have a buoyant density in sucrose solution of 1.21 to 1.23 g/ml. A single protein, recognized by our anti-microtubular aggregates monoclonal antibodies, was found to have an Mr of 44,000 (p44). This p44 protein was not found in uninfected chimpanzees. We determined a partial amino acid sequence for p44, and showed that it has no homology to any known proteins. |
| Databáze: | OpenAIRE |
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