Thyroid hormone receptor beta mutations in the 'hot-spot region' are rare events in thyroid carcinomas
| Autor: | Ana Rocha, Paula Soares, Inês Vieira de Castro, Ricardo Soares, J.F Magalhaes, Inês Bento, Ricardo Marques |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Electrophoresis
endocrine system medicine.medical_specialty endocrine system diseases Endocrinology Diabetes and Metabolism DNA Mutational Analysis Biology medicine.disease_cause Polymorphism Single Nucleotide Thyroid carcinoma Thyroid hormone receptor beta Endocrinology Internal medicine Cell Line Tumor medicine Humans Thyroid Neoplasms Thyroid cancer Thyroid hormone receptor Paraffin Embedding Thyroid Thyroid Hormone Receptors beta Exons medicine.disease Carcinoma Papillary medicine.anatomical_structure PAX8 Carcinogenesis V600E |
| Zdroj: | The Journal of endocrinology. 192(1) |
| ISSN: | 0022-0795 |
| Popis: | Thyroid cancer constitutes the most frequent endocrine neoplasia. Targeted expression of rearranged during transfection (RET)/papillary thyroid carcinoma (PTC) and V600E V-raf murine sarcoma viral oncogene homolog B1 (BRAF) to the thyroid glands of transgenic mice results in tumours similar to those of human PTC, providing evidence for the involvement of these oncogenes in PTC. Kato et al. developed a mouse model that mimics the full spectrum of the human follicular form of thyroid cancer (FTC). FTC rapidly develops in these mice through introduction of the thyroid hormone receptor β (THRB)PV mutant on the background of the inactivated THRB wt locus. Our aim was to verify if, in the context of human follicular thyroid carcinogenesis, THRB acted as a tumour suppressor gene. We screened for mutations of the THRB gene in the hot-spot region, spanning exons 7–10, in 51 thyroid tumours and six thyroid cancer cell lines by PCR and direct sequencing. We did not find mutations in any of the tumours or cell lines analysed. Our findings suggest that, in contrast to the findings on the THRB-mutant transgenic mice, THRB gene mutations are not a relevant mechanism for human thyroid carcinogenesis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|