Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance
| Autor: | Laura N. Watson, Jörg Stetefeld, Kevin L. Campbell, Angela M. Sloan, Anthony V. Signore, Michael Hofreiter, Jeremy J. Austin, Jesse W Howatt, Tong-Jian Shen, Roy E. Weber, Jason E E Roberts, Alan Cooper, Nadin Rohland, Chien Ho, Jeremy R. H. Tame |
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| Rok vydání: | 2010 |
| Předmět: |
Models
Molecular Woolly mammoth biology Fossils Cold tolerance Partial Pressure Elephants Molecular Sequence Data Amino acid substitution Diphosphoglyceric Acids biology.organism_classification Adaptation Physiological Molecular biology Cold Temperature Evolution Molecular Oxygen Hemoglobins Mammoths Amino Acid Substitution Biochemistry Genetics Animals Hemoglobin |
| Zdroj: | Campbell, K L, Roberts, J E E, Watson, L N, Stetefeld, J, Sloan, A M, Signore, A V, Howatt, J W, Tame, J R H, Rohland, N, Shen, T-J, Austin, J J, Hofreiter, M, Weber, R E & Cooper, A 2010, ' Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance ', Nature Genetics, vol. 42, pp. 536-540 . https://doi.org/10.1038/ng.574 |
| ISSN: | 1546-1718 1061-4036 |
| DOI: | 10.1038/ng.574 |
| Popis: | We have genetically retrieved, resurrected and performed detailed structure-function analyses on authentic woolly mammoth hemoglobin to reveal for the first time both the evolutionary origins and the structural underpinnings of a key adaptive physiochemical trait in an extinct species. Hemoglobin binds and carries O2; however, its ability to offload O2 to respiring cells is hampered at low temperatures, as heme deoxygenation is inherently endothermic (that is, hemoglobin-O2 affinity increases as temperature decreases). We identify amino acid substitutions with large phenotypic effect on the chimeric β/δ-globin subunit of mammoth hemoglobin that provide a unique solution to this problem and thereby minimize energetically costly heat loss. This biochemical specialization may have been involved in the exploitation of high-latitude environments by this African-derived elephantid lineage during the Pleistocene period. This powerful new approach to directly analyze the genetic and structural basis of physiological adaptations in an extinct species adds an important new dimension to the study of natural selection. |
| Databáze: | OpenAIRE |
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