Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living mice
| Autor: | X. Z. Lewis, S. Bhaumik, Sanjiv Gambhir |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Metabolic Clearance Rate
Biomedical Engineering Firefly Luciferin Kidney Isozyme Sensitivity and Specificity Cell Line Substrate Specificity Biomaterials chemistry.chemical_compound Mice Neuroblastoma Genes Reporter Coelenterazine Chlorocebus aethiops Bioluminescence Animals Humans Luciferase Tissue Distribution Luciferases Gene Reporter gene Imidazoles Kidney metabolism Reproducibility of Results Anatomy Glioma Anthozoa Molecular biology Atomic and Molecular Physics and Optics Electronic Optical and Magnetic Materials Rats Coleoptera Isoenzymes chemistry Microscopy Fluorescence Cell culture Organ Specificity Pyrazines COS Cells |
| Zdroj: | Journal of biomedical optics. 9(3) |
| ISSN: | 1083-3668 |
| Popis: | We have recently demonstrated that Renilla luciferase (Rluc) is a promising bioluminescence reporter gene that can be used for noninvasive optical imaging of reporter gene expression in living mice, with the aid of a cooled charged couple device (CCD) camera. In the current study, we explore the expression of a novel synthetic Renilla luciferase reporter gene (hRluc) in living mice, which has previously been reported to be a more sensitive reporter than native Rluc in mammalian cells. We explore the strategies of simultaneous imaging of both Renilla luciferase enzyme (RL) and synthetic Renilla luciferase enzyme (hRL):coelenterazine (substrate for RL/hRL) in the same living mouse. We also demonstrate that hRL:coelenterazine can yield a higher signal when compared to Firefly luciferase enzyme (FL): D-Luciferin, both in cell culture studies and when imaged from cells at the surface and from lungs of living mice. These studies demonstrate that hRluc should be a useful primary reporter gene with high sensitivity when used alone or in conjunction with other bioluminescence reporter genes for imaging in living rodents. |
| Databáze: | OpenAIRE |
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