Highly Efficient Trityl-nitroxide Biradicals for Biomolecular High-field Dynamic Nuclear Polarization
| Autor: | Cai, Xinyi, Paioni, Alessandra Lucini, Adler, Agnes, Yao, Ru, Zhang, Wenxiao, Beriashvili, David, Safeer, Adil, Gurinov, Andrei, Rockenbauer, Antal, Song, Yuguang, Baldus, Marc, Liu, Yangping, Sub NMR Spectroscopy, NMR Spectroscopy |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Nitroxide mediated radical polymerization
Magnetic Resonance Spectroscopy Globular protein polarizing agents chemical and pharmacologic phenomena Catalysis law.invention dynamic nuclear polarization law Polarization (electrochemistry) Spectroscopy Electron paramagnetic resonance solid-state NMR spectroscopy chemistry.chemical_classification Biomolecule Organic Chemistry Membrane Proteins General Chemistry Small molecule biradicals Magnetic Fields electron paramagnetic resonance Solid-state nuclear magnetic resonance chemistry Chemical physics Nitrogen Oxides |
| Zdroj: | Chemistry-A European Journal, 27(50), 12758. Wiley |
| ISSN: | 0947-6539 |
| Popis: | Dynamic nuclear polarization (DNP) is a powerful method to enhance the sensitivity of solid-state magnetic nuclear resonance (ssNMR) spectroscopy. However, its biomolecular applications at high magnetic fields (preferably > 14 T) have so far been limited by the intrinsically low efficiency of polarizing agents and sample preparation aspects. Herein, we report a new class of trityl-nitroxide biradicals, dubbed SNAPols that combine high DNP efficiency with greatly enhanced hydrophilicity. SNAPol-1, the best compound in the series, shows DNP enhancement factors at 18.8 T of more than 100 in small molecules and globular proteins and also exhibits strong DNP enhancements in membrane proteins and cellular preparations. By integrating optimal sensitivity and high resolution, we expect widespread applications of this new polarizing agent in high-field DNP/ssNMR spectroscopy, especially for complex biomolecules. |
| Databáze: | OpenAIRE |
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