Kinetics of lipase catalyzed isoamyl acetate synthesis at high hydrostatic pressure in hexane
| Autor: | José I. Reyes-De-Corcuera, Michael J. Eisenmenger |
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| Rok vydání: | 2010 |
| Předmět: |
Chromatography
biology Chemistry Kinetics Hydrostatic pressure Temperature Isoamyl acetate Triacylglycerol lipase Bioengineering Lipase General Medicine Applied Microbiology and Biotechnology Michaelis–Menten kinetics Catalysis Hexane chemistry.chemical_compound Pentanols Hydrostatic Pressure biology.protein Hexanes Biotechnology |
| Zdroj: | Biotechnology Letters. 32:1287-1291 |
| ISSN: | 1573-6776 0141-5492 |
| DOI: | 10.1007/s10529-010-0288-7 |
| Popis: | Lipase-catalyzed synthesis of isoamyl acetate in hexane at 10–250 MPa at 80°C and 1–100 MPa at 40°C resulted in activation volumes of −12.9 ± 1.7 and −21.6 ± 2.9 cm3 mol−1, respectively. Increasing pressure from 10 to 200 MPa resulted in approximately 10-fold increase in V max at both 40 and 80°C. Pressure increased the K m from 2.4 ± 0.004 to 38 ± 0.78 mM at 40°C. In contrast, at 80°C the pressure did not affect the K m. |
| Databáze: | OpenAIRE |
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