Detection and quantification of Covid-19 antiviral drugs in biological fluids and tissues
| Autor: | Maria Assunta Acquavia, Raffaella Pascale, Luca Foti, Laura Scrano, Antonia Nicolò, Vincenzo Brancaleone, Giuseppe Martelli, Tommaso R. I. Cataldi, Giuliana Bianco |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
HILIC
hydrophilic interaction liquid chromatography PIs protease inhibitors RSD relative standard deviation Review CE capillary electrophoresis MSI MALDI-MS Imaging 02 engineering and technology Chemical Fractionation Biological matrices Pharmacology CSF cerebrospinal fluid 01 natural sciences Analytical Chemistry Drug detection MRM Muliple Reaction Monitoring OSP oseltamivir phosphate Tandem Mass Spectrometry TDM therapeutic drug monitoring Ph. Eur European Pharmacopoeia Chromatography High Pressure Liquid ESI electrospray ionization LC liquid chromatography Chemistry FL fluorescence Antivirals 021001 nanoscience & nanotechnology RdRpIs RNA polymerase inhibitors LLE liquid-liquid extraction Err absolute value of the relative error 0210 nano-technology Q-MSI quantitative MALDI-MS Imaging RE relative error SDS sodium dodecyl sulfate Coronavirus disease 2019 (COVID-19) MALDI matrix-assisted laser desorption/ionization Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) PSA primary-secondary amine Liquid chromatography HLB hydrophilic-lipophilic balance LLOQ lower limit of quantification Q QuEChERS TCA trichloroacetic acid Quechers Antiviral Agents CHCA α-cyano-4-hydroxycinnamic acid BeWos human placental choriocarcinoma trophobplast cells Capillary electrophoresis Pharmacokinetics Analytical methods UAE ultrasound assisted extraction PMA phosphomolybdic acid Biological fluids Animals Humans ADME adsorption distribution metabolism and elimination ICH International Conference on Harmonization Pandemics SRM Selected Reaction Monitoring PBA phenylboronic acid LOQ limit of quantification PBMCs peripheral blood mononuclear cells UHPLC ultra-high performance liquid chromatography LOD limit of detection Mass spectrometry SARS-CoV-2 SPE solid-phase extraction 010401 analytical chemistry d-SPE dispersive-sold-phase extraction COVID-19 CV coefficient of variation MTBE methyltertbutyl ether ACN acetonitrile FDA Food and Drug Administration COVID-19 Drug Treatment 0104 chemical sciences MCX mixed-mode cationic exchange QC quality control Clinical trial NRTIs nucleoside reverse transcriptase inhibitors PP protein precipitation MS mass spectrometry MLC micellar liquid chromatography CRMs certified reference materials DEV deviation from nominal concentration value EMA European Medicines Agency AD absolute deviation DHB 2 5-dihydroxybenzoic acid |
| Zdroj: | Talanta |
| ISSN: | 0039-9140 |
| DOI: | 10.1016/j.talanta.2020.121862 |
| Popis: | Since coronavirus disease 2019 (COVID-19) started as a fast-spreading pandemic, causing a huge number of deaths worldwide, several therapeutic options have been tested to counteract or reduce the clinical symptoms of patients infected with the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Currently, no specific drugs for COVID-19 are available, but many antiviral agents have been authorised by several national agencies. Most of them are under investigation in both preclinical and clinical trials; however, pharmacokinetic and metabolism studies are needed to identify the most suitable dose to achieve the desired effect on SARS-CoV-2. Therefore, the efforts of the scientific community have focused on the screening of therapies able to counteract the most severe effects of the infection, as well as on the search of sensitive and selective analytical methods for drug detection in biological matrices, both fluids and tissues. In the last decade, many analytical methods have been proposed for the detection and quantification of antiviral compounds currently being tested for COVID-19 treatment. In this review, a critical discussion on the overall analytical procedure is provided, i.e (a) sample pre-treatment and extraction methods such as protein precipitation (PP), solid-phase extraction (SPE), liquid–liquid extraction (LLE), ultrasound-assisted extraction (UAE) and QuEChERS (quick, easy, cheap, effective, rugged and safe), (b) detection and quantification methods such as potentiometry, spectrofluorimetry and mass spectrometry (MS) as well as (c) methods including a preliminary separation step, such as high performance liquid chromatography (HPLC) and capillary electrophoresis (CE) coupled to UV–Vis or MS detection. Further current trends, advantages and disadvantages and prospects of these methods have been discussed, to help the analytical advances in reducing the harm caused by the SARS-CoV-2 virus. Graphical abstract Image 1 Highlights • Fourteen antiviral drugs were tested to counteract the effects of COVID-19. • A review of analytical methods for antivirals detection is presented. • Method validation, drugs extraction, separation and detection are discussed. • LC-MS and MS/MS is mostly used for accurate and sensitive drugs quantification. |
| Databáze: | OpenAIRE |
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