Mucosal antibody responses to vaccines targeting SIV protease cleavage sites or full-length Gag and Env proteins in Mauritian cynomolgus macaques
| Autor: | José Crecente-Campo, María J. Alonso, Hongzhao Li, Ma Luo, Francis A. Plummer, Mohammad Abul Kashem, Lin Li, Dane Schalk, Lewis R. Liu, Robert W Omange, Nikki Toledo, Binhua Liang, James B. Whitney, Qingsheng Li, Yan Hai, Nancy Schultz-Darken, Tamara G Dacoba, So-Yon Lim, Eva G. Rakasz, Yanmin Wan |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Centro de Investigación en Medicina Molecular e Enfermidades Crónicas, Universidade de Santiago de Compostela. Departamento de Farmacoloxía, Farmacia e Tecnoloxía Farmacéutica, University of Manitoba |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
viruses T cell Mauritian cynomolgus macaques lcsh:Medicine Gene Products gag Monkeys Cross Reactions Biology Antibodies Viral Cleavage (embryo) 03 medical and health sciences 0302 clinical medicine Immune system Western blot medicine Animals Amino Acid Sequence 030212 general & internal medicine HIV vaccine lcsh:Science Protease cleavage sites Binding Sites Multidisciplinary Innate immune system medicine.diagnostic_test lcsh:R SAIDS Vaccines Gene Products env virus diseases Virology Macaca fascicularis 030104 developmental biology medicine.anatomical_structure Full-length Gag and Env Proteolysis biology.protein lcsh:Q Female Immunization Simian Immunodeficiency Virus Mucosal antibodies Mucosal antibody Antibody Vaccine Peptide Hydrolases |
| Zdroj: | PLoS ONE, Vol 13, Iss 8, p e0202997 (2018) Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| ISSN: | 1932-6203 |
| Popis: | HIV mutates rapidly and infects CD4+ T cells, especially when they are activated. A vaccine targeting conserved, essential viral elements while limiting CD4+ T cell activation could be effective. Learning from natural immunity observed in a group of highly HIV-1 exposed seronegative Kenyan female sex workers, we are testing a novel candidate HIV vaccine targeting the 12 viral protease cleavage sites (PCSs) (the PCS vaccine), in comparison with a vaccine targeting full-length Gag and Env (the Gag/Env vaccine) in a Mauritian cynomolgus macaque/SIV model. In this study we evaluated these vaccines for induction of mucosal antibodies to SIV immunogens at the female genital tract. Bio-Plex and Western blot analyses of cervicovaginal lavage samples showed that both the PCS and Gag/Env vaccines can elicit mucosal IgG antibody responses to SIV immunogens. Significantly higher increase of anti-PCS antibodies was induced by the PCS vaccine than by the Gag/Env vaccine (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|