Localized hydrogel delivery of dendritic cells for attenuation of multiple sclerosis in a murine model
| Autor: | Nicholas M. Beskid, Aline M. Thomas, Julia E. Babensee, Aaron M. Rosado, Brian D. Evavold, Jennifer Lori Blanchfield, Andrés J. García |
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| Rok vydání: | 2020 |
| Předmět: |
Materials science
Encephalomyelitis Autoimmune Experimental Multiple Sclerosis 0206 medical engineering Cell Central nervous system Biomedical Engineering 02 engineering and technology Polyethylene Glycols Biomaterials Myelin Mice Immune system medicine Animals Cells Cultured Tissue Scaffolds Multiple sclerosis Experimental autoimmune encephalomyelitis Metals and Alloys Hydrogels Dendritic Cells 021001 nanoscience & nanotechnology medicine.disease 020601 biomedical engineering Transplantation Mice Inbred C57BL Disease Models Animal medicine.anatomical_structure Cervical lymph nodes Ceramics and Composites Cancer research Female 0210 nano-technology |
| Zdroj: | Journal of biomedical materials research. Part AREFERENCES. 109(7) |
| ISSN: | 1552-4965 |
| Popis: | In multiple sclerosis (MS), abnormally activated immune cells responsive to myelin proteins result in widespread damage throughout the central nervous system (CNS) and ultimately irreversible disability. Immunomodulation by delivering dendritic cells (DCs) utilizes a potent and rapid MS disease progression driver therapeutically. Here, we investigated delivering DCs for disease severity attenuation using an experimental autoimmune encephalomyelitis preclinical MS model. DCs treated with interleukin-10 (IL-10) (DC10s) were transplanted using in situ gelling poly(ethylene glycol)-based hydrogel for target site localization. DC delivery increased hydrogel longevity and altered the injection site recruited, endogenous immune cell profile within 2 days postinjection. Furthermore, hydrogel-mediated DC transplantation efficacy depended on the injection-site. DCs delivered to the neck local to MS-associated CNS-draining cervical lymph nodes attenuated paralysis, compared to untreated controls, while delivery to the flank did not alter paralysis severity. This study demonstrates that local delivery of DC10s modulates immune cell recruitment and attenuates disease progression in a preclinical model of MS. |
| Databáze: | OpenAIRE |
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