Altered Cyclooxygenase-2 Expression in Pulmonary Sarcoidosis is not Related to Clinical Classifications

Autor: Ewa Brzeziańska-Lasota, Monika Migdalska-Sęk, Daria Domańska-Senderowska, Paweł Górski, Witold Górski, Ewa Nawrot, Karolina H. Czarnecka, Dorota Pastuszak-Lewandoska, Adam Antczak, Justyna Kiszałkiewicz, Wojciech J. Piotrowski
Rok vydání: 2016
Předmět:
Zdroj: Inflammation. 39:1302-1309
ISSN: 1573-2576
0360-3997
DOI: 10.1007/s10753-016-0362-y
Popis: Elevated COX-2 activity is associated with the development of chronic lung diseases leading to bronchial obstruction, including sarcoidosis. The aim of the study was to examine expression pattern of COX-2 messenger RNA (mRNA). Expression was performed by q-PCR method in bronchoalveolar lavage (BAL) cells and peripheral blood (PB) lymphocytes in sarcoidosis patients (n = 61) and control group (n = 30). Analysis of COX-2 mRNA expression level in BAL fluid and PB revealed downregulation in sarcoidosis and control groups. In PB lymphocytes, the statistically significant difference between patients and controls was observed (P = 0.003, Mann–Whitney U test), with higher expression in patients. There were no statistically significant differences between patients without and with parenchymal involvement (stages I vs. II–IV), between patients with acute vs. insidious onset of disease and between patients with abnormal vs. normal spirometry (P > 0.05, Mann–Whitney U test). Results suggest that expression of COX-2 mRNA in patients with pulmonary sarcoidosis is not related to clinical classifications.
Databáze: OpenAIRE
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