Diosgenin Loaded Polymeric Nanoparticles with Potential Anticancer Efficacy
| Autor: | Monisha Singhal, R. Mankamna Kumari, Surendra Nimesh, Nikita Sharma, Asad Syed, Romila Manchanda, Nidhi Gupta, Ali H. Bahkali |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Glycerol
Light Cell Survival Polymers Malates lcsh:QR1-502 Nanoparticle Antineoplastic Agents Apoptosis PGMD nanoparticles 02 engineering and technology In Vitro Techniques anticancer Biochemistry Article lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound Inhibitory Concentration 50 0302 clinical medicine Drug Delivery Systems Dynamic light scattering Ethidium Humans Scattering Radiation Particle Size Molecular Biology Dissolution IC50 Drug Carriers Chemistry Diosgenin Models Theoretical 021001 nanoscience & nanotechnology Polymeric nanoparticles Box–Behnken design In vitro Acridine Orange Dynamic Light Scattering Drug Liberation Kinetics A549 Cells 030220 oncology & carcinogenesis diosgenin Nanoparticles Box-Behnken design 0210 nano-technology Nuclear chemistry |
| Zdroj: | Biomolecules, Vol 10, Iss 1679, p 1679 (2020) Biomolecules Volume 10 Issue 12 |
| Popis: | This study aims to determine the anticancer efficacy of diosgenin encapsulated poly-glycerol malate co-dodecanedioate (PGMD) nanoparticles. Diosgenin loaded PGMD nanoparticles (variants 7:3 and 6:4) were synthesized by the nanoprecipitation method. The synthesis of PGMD nanoparticles was systematically optimized employing the Box-Behnken design and taking into account the influence of various independent variables such as concentrations of each PGMD, diosgenin and PF-68 on the responses such as size and PDI of the particles. Mathematical modeling was done using the Quadratic second order modeling method and response surface analysis was undertaken to elucidate the factor-response relationship. The obtained size of PGMD 7:3 and PGMD 6:4 nanoparticles were 133.6 nm and 121.4 nm, respectively, as measured through dynamic light scattering (DLS). The entrapment efficiency was in the range of 77&ndash 83%. The in vitro drug release studies showed diffusion and dissolution controlled drug release pattern following Korsmeyer&ndash Peppas kinetic model. Furthermore, in vitro morphological and cytotoxic studies were performed to evaluate the toxicity of synthesized drug loaded nanoparticles in model cell lines. The IC50 after 48 h was observed to be 27.14 µ M, 15.15 µ M and 13.91 µ M for free diosgenin, PGMD 7:3 and PGMD 6:4 nanoparticles, respectively, when administered in A549 lung carcinoma cell lines. |
| Databáze: | OpenAIRE |
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