Spatial association of apoptosis-related gene expression and cellular death in clinical neuroblastoma
| Autor: | J. C. Hoehner, L Olsen, Carolina Gestblom, Sven Påhlman |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
Cancer Research Programmed cell death Tumor suppressor gene Cellular differentiation Apoptosis Biology Neuroblastoma Stroma medicine Humans Proliferation Marker Fragmentation (cell biology) Child Cell Death Infant Newborn Infant Cell Differentiation Ganglioneuroma Genes p53 medicine.disease Immunohistochemistry Genes bcl-2 Proliferating cell nuclear antigen Gene Expression Regulation Neoplastic Oncology Child Preschool Cancer research biology.protein Female Cell Division Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1997.203 |
| Popis: | Several unique features of neuroblastoma (NB), including the capacity for spontaneous regression and maturation to benign pathology, suggest that genes that regulate cellular proliferation, survival and differentiation may be involved in directing clinical tumour aggressiveness. The in situ expression of Bcl-2, Rb, p21, p53 and Bax proteins, as well as the proliferation marker proliferating cell nuclear antigen (PCNA) were examined immunocytochemically in a selection of 38 stage- and outcome-identified NB tumours. Apoptotic cells were identified morphologically and by a DNA fragmentation labelling technique (TUNEL). Although the tumour cell density of Bcl-2, p53, Bax, PCNA and TUNEL positivity correlated with patient survival, a spatially organized expression pattern was further recognized in stroma-poor differentiating tumours. Immature tumour cells adjacent to thin fibrovascular stroma are proliferating, as evidenced by PCNA positivity, and often express Bcl-2. At increasing distance from this fibrovascular stroma, intermediately differentiated tumour cells express Rb, while with more advanced differentiation, proliferation ceases and Bcl-2 immunoreactivity is lost. The most differentiated tumour cells, which often express p53, and occasionally p21 and Bax, lie adjacent to TUNEL-positive, morphologically apoptotic cells. This spatial organization in favourable outcome NB tumours suggests that physiological regulation of differentiation and apoptosis may be involved in tumour regression. Images Figure 2 |
| Databáze: | OpenAIRE |
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