A novel ratiometric fluorescent approach for the modulation of the dynamic range of lateral flow immunoassays
| Autor: | Amadeo Sena‐Torralba, Helena Torné‐Morató, Claudio Parolo, Saba Ranjbar, Mohammad Amin Farahmand Nejad, Ruslan Álvarez‐Diduk, Andrea Idili, Mohammad Reza Hormozi‐Nezhad, Arben Merkoçi |
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| Přispěvatelé: | Ministerio de Economía y Competitividad (España), European Commission, Generalitat de Catalunya, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), European Research Council, Agence Nationale de la Recherche (France) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
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| Popis: | The majority of lateral flow assays (LFAs) use single-color optical labels to provide a qualitative naked-eye detection, however this detection method displays two important limitations. First, the use of a single-color label makes the LFA prone to results misinterpretation. Second, it does not allow the precise modulation of the sensitivity and dynamic range of the test. To overcome these limitations, a ratiometric approach is developed. In particular, using anti-HIgG functionalized red-fluorescent quantum dots on the conjugate pad (as target dependent labels) and blue-fluorescent nanoparticles fixed on the test line (as target independent reporters), it is possible to generate a wide color palette (blue, purple, pink, red) on the test line. It is believed that this strategy will facilitate the development of LFAs by easily adjusting their analytical properties to the needs required by the specific application. The authors acknowledge financial support from NACANCELL project PCIN-2016-066 (program Euronanomed 2). This work was also funded by the CERCA Program/Generalitat de Catalunya. The ICN2 was funded by the CERCA program/Generalitat de Catalunya. ICN2 acknowledges the support of the Spanish MINECO for the Project MAT2017-87202-P and through the Severo Ochoa Centres of Excellence Program under Grant SEV2201320295. A.I. was supported by PROBIST postdoctoral fellowship funded by European Research Council (Marie Sklodowska Curie) grant agreement No. 754510). C.P. acknowledges support from the Spanish Ministry of Science and Innovation and State Research Agency through the “Centro de Excelencia Severo Ochoa 2019-2023” Program (CEX2018-000806-S), and support from the Generalitat de Catalunya through the CERCA Program. |
| Databáze: | OpenAIRE |
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