Dissemination of pHK01-like incompatibility group IncFII plasmids encoding CTX-M-14 in Escherichia coli from human and animal sources
| Autor: | Susan S. Chiu, Pak-Leung Ho, Amy Hin Yan Tong, Zhen Li, Jessie Y.J. Bao, Wai-U Lo, Chi-Ho Lin, Si Lok, Jane Chan, M. K. Yeung, Wing-Cheong Yam, Kin-Hung Chow |
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| Rok vydání: | 2012 |
| Předmět: |
Adult
Urine Biology medicine.disease_cause Microbiology beta-Lactamases Feces Plasmid Polymorphism (computer science) Escherichia coli medicine Animals Humans Typing Replicon Child Gene Escherichia coli Infections Molecular Epidemiology General Veterinary Molecular epidemiology Escherichia coli Proteins General Medicine Subtyping Female Plasmids |
| Zdroj: | Veterinary Microbiology. 158:172-179 |
| ISSN: | 0378-1135 |
| DOI: | 10.1016/j.vetmic.2012.02.004 |
| Popis: | Few studies have compared CTX-M encoding plasmids identified in different ecological sources. This study aimed to analyze and compare the molecular epidemiology of plasmids encoding CTX-M-14 among strains from humans and animals. The CTX-M-14 encoding plasmids in 160 Escherichia coli isolates from animal faecal (14 pigs, 16 chickens, 12 cats, 8 cattle, 5 dogs and 3 rodents), human faecal (45 adults and 20 children) and human urine (37 adults) sources in 2002-2010 were characterized by molecular methods. The replicon types of the CTX-M-14 encoding plasmids were IncFII (n=61), I1-Iγ (n=24), other F types (n=23), B/O (n=10), K (n=6), N (n=3), A/C (n=1), HI1 (n=1), HI2 (n=1) and nontypeable (n=30). The genetic environment, ISEcp1 -bla(CTX-M-14) - IS903 was found in 89.7% (52/58), 87.7% (57/65) and 86.5% (32/37) of the animal faecal, human faecal and human urine isolates, respectively. Subtyping of the 61 IncFII incompatibility group plasmids by replicon sequence typing, plasmid PCR-restriction fragment length polymorphism and marker genes (yac, malB, eitA/eitC and parB/A) profiles showed that 31% (18/58), 30.6% (20/65) and 37.8% (14/37) of the plasmids originating from animal faecal, human faecal and human urine isolates, respectively, were pHK01-like. These 52 pHK01-like plasmids originated from diverse human (20 faecal isolates from 2002, 2007 to 2008, 14 urinary isolates from 2004) and animal (all faecal, 1 cattle, 1 chicken, 5 pigs, 9 cats, 1 dog, 1 rodent from 2008 to 2010) sources. In conclusion, this study highlights the importance of the IncFII group, pHK01-like plasmids in the dissemination of CTX-M-14 among isolates from diverse sources. |
| Databáze: | OpenAIRE |
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