Identification of secreted proteins of Aspergillus oryzae associated with growth on solid cereal substrates
| Autor: | Amandine Boussier, R. te Biesebeke, N. van Biezen, Peter J. Punt, C.A.M.J.J. van den Hondel |
|---|---|
| Přispěvatelé: | TNO Kwaliteit van Leven |
| Rok vydání: | 2005 |
| Předmět: |
Hyphal growth
cereal Aspergillus oryzae fungal protein xylan endo 1 3 beta xylosidase Applied Microbiology and Biotechnology chemistry.chemical_compound Microbiologie Amylase chemistry.chemical_classification Growth medium Fungal protein biology article food and beverages General Medicine alpha arabinofuranosidase Enzymes Biochemistry priority journal chitinase Xylanase Biotechnology amylase culture medium Triticum aestivum Cereals Bioengineering Microbiology Fungal Proteins protein secretion Filamentous Biology VLAG nonhuman Substrates Fungi sequence homology biology.organism_classification state fermentation fungus growth Enzyme chemistry protein analysis Chitinase Cereal substrates biology.protein peptides amino terminal sequence Cell culture Edible Grain |
| Zdroj: | Journal of Biotechnology, 4, 121, 482-485 Journal of Biotechnology, 121(4), 482-485 Journal of Biotechnology 121 (2006) 4 |
| ISSN: | 0168-1656 |
| Popis: | Filamentous growth of Aspergillus oryzae on solid cereal substrates involves secretion of substrate converting enzymes and a solid substrate specific polarised hyphal growth phenotype. To identify proteins produced under these specific conditions, the extracts of A. oryzae grown on wheat-based media were analysed using N-terminal sequence analysis. In a submerged wheat-based growth medium of A. oryzae, besides α-amylase, also an arabinosidase and xylanase were abundantly produced. In the extracts of A. oryzae grown on wheat-based solid substrate besides α-amylase and chitinase, two new proteins of 16 and 27 kDa were identified. These hypothetical proteins showed only close homologies to filamentous fungal proteins. © 2005 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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