Molecular characterization of Nipah virus from Pteropus hypomelanus in Southern Thailand
| Autor: | Panumas Samseeneam, Thiravat Hemachudha, Mana Phermpool, Supaporn Wacharapluesadee, Budsabong Kanchanasaka, Phimchanok Srongmongkol, Pattarapol Maneeorn, Thongchai Kaewpom, Apaporn Rodpan |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Veterinary medicine Plastic sheet Nipah virus 030231 tropical medicine Short Report Urine Polymerase Chain Reaction Strain law.invention 03 medical and health sciences 0302 clinical medicine law Chiroptera Virology Bats parasitic diseases Transmission Animals Viral rna Nucleocapsid Southern Phylogeny Polymerase chain reaction biology Nipah Virus Genetic Variation Outbreak Sequence Analysis DNA Thailand Pteropus biology.organism_classification 030104 developmental biology Infectious Diseases RNA Viral |
| Zdroj: | Virology Journal |
| ISSN: | 1743-422X |
| Popis: | Background Nipah virus (NiV) first emerged in Malaysia in 1998, with two bat species (Pteropus hypomelanus and P. vampyrus) as the putative natural reservoirs. In 2002, NiV IgG antibodies were detected in these species from Thailand, but viral RNA could not be detected for strain characterization. Two strains of NiV (Malaysia and Bangladesh) have been found in P. lylei in central Thailand, although Bangladesh strain, the causative strain for the outbreak in Bangladesh since 2001, was dominant. To understand the diversity of NiV in Thailand, this study identified NiV strain, using molecular characterizations, from P. hypomelanus in southern Thailand. Findings Pooled bat urine specimens were collected from plastic sheet underneath bat roosts in April 2010, and then monthly from December 2010 to May 2011 at an island in southern Thailand. Five in 184 specimens were positive for NiV, using duplex nested RT-PCR assay on partial nucleocapsid fragment (357 bp). Whole sequences of nucleocapsid gene from four bats were characterized. All 5 partial fragments and 4 whole nucleocapsid genes formed a monophyletic with NiV-MY. Conclusions Our study showed that P. hypomelanus in southern Thailand and from Malaysia, a bordering country, harbored similar NiV. This finding indicates that NiV is not limited to central Thailand or P. lylei species, and it may be a source of inter-species transmission. This indicates a higher potential for a widespread NiV outbreak in Thailand. NiV surveillance in Pteropus bats, the major natural reservoirs, should be conducted continuously in countries or regions with high susceptibility to outbreaks. |
| Databáze: | OpenAIRE |
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