Identification of Antibody Against SNRPB, Small Nuclear Ribonucleoprotein-Associated Proteins B and B', as an Autoantibody Marker in Crohn's Disease using an Immunoproteomics Approach
| Autor: | Garrick Wallstrom, Joshua LaBaer, Ji Qiu, Kristi Barker, Kailash Karthikeyan, Yanyang Tang, Gokhan Demirkan, Haoyu Wang, Xiaofang Bian, Shabana F. Pasha, Jonathan A. Leighton |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Immunoglobulin A Adult Male Proteomics Adolescent Peripherins Protein Array Analysis Sensitivity and Specificity Immunoproteomics Immunoglobulin G Antibodies snRNP Core Proteins 03 medical and health sciences Random Allocation Young Adult Crohn Disease medicine Humans Aged Crohn's disease biology business.industry Gastroenterology Autoantibody General Medicine Middle Aged medicine.disease Securin 030104 developmental biology Case-Control Studies Immunology biology.protein Biomarker (medicine) Female Snail Family Transcription Factors Antibody business Small nuclear ribonucleoprotein Biomarkers |
| Zdroj: | Journal of Crohn'scolitis. 11(7) |
| ISSN: | 1876-4479 |
| Popis: | Background Current non-invasive biomarkers for Crohn's disease are limited in their utility. Progress in identifying individual autoantigens and autoantibodies in Crohn's disease has been challenging due to limitations of available immunoassays. Aims Our aim was to identify autoantibodies associated with Crohn's disease that may be useful in diagnosis and management using an innovative protein array technology, namely nucleic acid programmable protein arrays [NAPPA]. Methods Serum samples of 96 patients with established Crohn's disease and 96 healthy controls were included and evenly split into discovery and validation sets randomly. Autoantibodies of both IgG and IgA classes were profiled against ~1900 human proteins in the discovery set on NAPPA. Autoantibodies discovered to be Crohn's disease-specific were further validated in the independent validation set by enzyme-linked immunosorbent assay. Results Overall, reactivity of IgG autoantibodies was stronger than that of IgA autoantibodies; however, IgA autoantibodies showed greater differential reactivity between cases and controls. Four IgA autoantibodies against SNRPB, PRPH, PTTG1 and SNAI1 were newly identified with sensitivities above 15% at 95% specificity, among which anti-SNRPB-IgA had the highest sensitivity of 24.0%. Autoantibodies associated with specific disease subtypes were also found. Conclusions As one of the first studies to use immunoproteomics for the identification of autoantibodies in Crohn's disease, our results support the utility of NAPPA in implementing future expanded studies with better coverage of the human proteome and microbial proteomes relevant to Crohn's disease and identifying antibody markers that may have clinical impact in diagnosis and management. |
| Databáze: | OpenAIRE |
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