PCR-RFLP analysis of the flagellin sequences for identification ofBurkholderia pseudomalleiandBurkholderia cepaciafrom clinical isolates
| Autor: | S. Tunpiboonsak, Sakol Panyim, Sumet Wajanarogana, Sumalee Tungpradabkul |
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| Rok vydání: | 1999 |
| Předmět: |
DNA
Bacterial Genetic Markers Burkholderia pseudomallei Molecular Sequence Data Sequence Homology Burkholderia cepacia Polymerase Chain Reaction law.invention Restriction fragment Microbiology Species Specificity law Humans Amino Acid Sequence Cloning Molecular Molecular Biology Polymerase chain reaction Genetics biology Burkholderia Infections Sequence Analysis DNA Cell Biology biology.organism_classification Restriction enzyme Burkholderia Genes Bacterial biology.protein bacteria Amplified fragment length polymorphism Restriction fragment length polymorphism Sequence Alignment Polymorphism Restriction Fragment Length Flagellin |
| Zdroj: | Molecular and Cellular Probes. 13:99-105 |
| ISSN: | 0890-8508 |
| DOI: | 10.1006/mcpr.1999.0221 |
| Popis: | The flagellin genes of four Burkholderia pseudomallei and two Burkholderia cepacia clinical isolates were studied by a polymerase chain reaction (PCR)-based isolation method using the same pair of primers. The PCR-amplification products of the isolates showed a single band of about 1·1 kb, which is similar to a type II B. cepacia flagellin reported previously. In order to distinguish these two species based on the flagellin PCR-amplified products, Pst I and Xho I restriction endonuclease analysis was performed. The results suggest that there is sufficient diversity within the flagellin sequences of the closely related Burkholderia species, B. pseudomallei and B. cepacia , to enable flagellin-type identification on the basis of the pattern of restriction fragments. In addition, the flagellin gene should be of considerable use as a genetic marker for clinical identification of these organisms. |
| Databáze: | OpenAIRE |
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