Immunosuppressive Effects of Mesenchymal Stem Cells: Involvement of HLA-G
| Autor: | Alain Chapel, Asma Boutarfa, Noëlle Mathieu, Sabine François, Christelle Mazurier, Sandrine Bouchet, N-Claude Gorin, Johanna Frick, Dominique Thierry, Aisha Nasef, Loic Fouillard |
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| Přispěvatelé: | CHU Saint-Antoine [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Interactions hôte-greffon-tumeur, ingénierie cellulaire et génique - UFC (UMR INSERM 1098) (RIGHT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang [Bourgogne-Franche-Comté] (EFS [Bourgogne-Franche-Comté])-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Saas, Philippe |
| Rok vydání: | 2007 |
| Předmět: |
T-Lymphocytes
Fluorescent Antibody Technique Gene Expression Graft vs Host Disease MESH: Flow Cytometry Lymphocyte proliferation MESH: Histocompatibility Antigens Class I 0302 clinical medicine HLA Antigens MESH: Reverse Transcriptase Polymerase Chain Reaction HLA-G MESH: Fluorescent Antibody Technique MESH: HLA Antigens Cells Cultured 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction MESH: Bone Marrow Cells MESH: Immunosuppression MESH: Enzyme-Linked Immunosorbent Assay Flow Cytometry 3. Good health 030220 oncology & carcinogenesis [SDV.IMM]Life Sciences [q-bio]/Immunology Stem cell MESH: Cells Cultured MESH: Gene Expression [SDV.IMM] Life Sciences [q-bio]/Immunology MESH: Graft vs Host Disease Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Human leukocyte antigen Mesenchymal Stem Cell Transplantation Major histocompatibility complex 03 medical and health sciences Antigen MESH: Cell Proliferation Humans MESH: Mesenchymal Stem Cell Transplantation RNA Messenger Cell Proliferation MESH: RNA Messenger 030304 developmental biology HLA-G Antigens Immunosuppression Therapy Transplantation MESH: Humans Histocompatibility Antigens Class I Mesenchymal stem cell Mesenchymal Stem Cells Histocompatibility MESH: Mesenchymal Stem Cells MESH: T-Lymphocytes Immunology biology.protein |
| Zdroj: | Transplantation Transplantation, Lippincott, Williams & Wilkins, 2007, 84 (2), pp.231-7. ⟨10.1097/01.tp.0000267918.07906.08⟩ |
| ISSN: | 0041-1337 1534-6080 |
| Popis: | International audience; INTRODUCTION: Mesenchymal stem cells (MSCs) possess unique immunomodulatory properties. They are able to suppress allogenic T-cell response and modify maturation of antigen-presenting cells. Their role in the treatment of severe graft versus host disease has been reported. The underlying molecular mechanisms of immunosuppression are currently being investigated. Histocompatibility locus antigen (HLA)-G is a nonclassical major histocompatibility complex class I antigen with strong immune-inhibitory properties. METHODS: We studied the role of HLA-G on MSC-induced immunosuppression. The expression of HLA-G on human MSCs cultured alone and in mixed lymphocytes reaction (MSC/MLR) was analyzed. RESULTS: We found that HLA-G can be detected on MSCs by real-time reverse-phase polymerase chain reaction, immunofluorescence, flow cytometry (52.4+/-3.6%), and enzyme-linked immunosorbent assay in the supernatant (38.7+/-5.2 ng/mL). HLA-G protein expression is constitutive and the level is not modified upon stimulation by allogenic lymphocytes in MSC/MLR. The functional role of HLA-G protein expressed by MSCs was analyzed using the 87G anti-HLA-G blocking antibody in a MSC/MLR. We found that blocking HLA-G molecule significantly raised lymphocyte proliferation in MSC/MLR (35.5%, P=0.01). CONCLUSION: Our findings provide evidences supporting involvement of HLA-G in the immunosuppressive properties of MSCs. These results emphasize the potential application of MSCs as a relevant therapeutic candidate in transplantation. |
| Databáze: | OpenAIRE |
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