Altering the insertional specificity of a Drosophila transposable element
| Autor: | Norbert Perrimon, E Noll, W F Odenwald, J A Kassis, E P VanSickle |
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| Předmět: |
Transposable element
Genetics Multidisciplinary biology Base Sequence Molecular Sequence Data biology.organism_classification engrailed Insert (molecular biology) P element Segmentation gene Drosophila melanogaster Enhancer Elements Genetic Gene Expression Regulation Genes Oligodeoxyribonucleotides Regulatory sequence Drosophilidae DNA Transposable Elements Animals Promoter Regions Genetic Research Article |
| Zdroj: | Scopus-Elsevier |
| Popis: | Vectors derived from the Drosophila P element transposon are widely used to make transgenic Drosophila. Insertion of most P-element-derived vectors is nonrandom, but they exhibit a broad specificity of target sites. During experiments to identify cis-acting regulatory elements of the Drosophila segmentation gene engrailed, we identified a fragment of engrailed DNA that, when included within a P-element vector, strikingly alters the specificity of target sites. P-element vectors that contain this fragment of engrailed regulatory DNA insert at a high frequency near genes expressed in stripes. |
| Databáze: | OpenAIRE |
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