Patch-seq: Past, Present, and Future
| Autor: | Dmitry Kobak, Cathryn R. Cadwell, Shreejoy J. Tripathy, Cedric Bardy, Kristen Hadley, Marcela Lipovsek |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Computer science Cells 1.1 Normal biological development and functioning Multimodal data multi-modal Computational biology Molecular neuroscience Medical and Health Sciences transcriptomics 03 medical and health sciences Functional diversity 0302 clinical medicine Underpinning research Genetics Animals Humans Anatomic Location Cells Cultured Brain function Neurons Cultured Neurology & Neurosurgery Sequence Analysis RNA neuronal morphology General Neuroscience Psychology and Cognitive Sciences Neurosciences patch-clamp electrophysiology single cell Electrophysiological Phenomena 030104 developmental biology MRNA Sequencing Neurological RNA Single-Cell Analysis Erratum Transcriptome Sequence Analysis 030217 neurology & neurosurgery Biotechnology Forecasting |
| Zdroj: | J Neurosci The Journal of neuroscience : the official journal of the Society for Neuroscience, vol 41, iss 5 |
| ISSN: | 1529-2401 0270-6474 |
| Popis: | Single-cell transcriptomic approaches are revolutionizing neuroscience. Integrating this wealth of data with morphology and physiology, for the comprehensive study of neuronal biology, requires multiplexing gene expression data with complementary techniques. To meet this need, multiple groups in parallel have developed “Patch-seq,” a modification of whole-cell patch-clamp protocols that enables mRNA sequencing of cell contents after electrophysiological recordings from individual neurons and morphologic reconstruction of the same cells. In this review, we first outline the critical technical developments that enabled robust Patch-seq experimental efforts and analytical solutions to interpret the rich multimodal data generated. We then review recent applications of Patch-seq that address novel and long-standing questions in neuroscience. These include the following: (1) targeted study of specific neuronal populations based on their anatomic location, functional properties, lineage, or a combination of these factors; (2) the compilation and integration of multimodal cell type atlases; and (3) the investigation of the molecular basis of morphologic and functional diversity. Finally, we highlight potential opportunities for further technical development and lines of research that may benefit from implementing the Patch-seq technique. As a multimodal approach at the intersection of molecular neurobiology and physiology, Patch-seq is uniquely positioned to directly link gene expression to brain function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|