The human actin-related protein hArp5: Nucleo-cytoplasmic shuttling and involvement in DNA repair

Autor: Mariko Kamo, Takafumi Uchida, Ryo Matsuda, Tsuyoshi Ikura, Barbara Winsor, Satoshi Tashiro, Yukako Oma, Kumiko Kitayama, Masahiko Harata, Takashi Ohyama
Rok vydání: 2009
Předmět:
Cytoplasm
Saccharomyces cerevisiae Proteins
DNA Repair
DNA repair
Recombinant Fusion Proteins
Nuclear Localization Signals
Active Transport
Cell Nucleus
Receptors
Cytoplasmic and Nuclear
Cell Cycle Proteins
Ataxia Telangiectasia Mutated Proteins
Karyopherins
Protein Serine-Threonine Kinases
Biology
Chromatin remodeling
Cell Line
Histones
Bleomycin
chemistry.chemical_compound
Transformation
Genetic
medicine
Humans
DNA Breaks
Double-Stranded
Angiopoietin-Like Protein 6
Phosphorylation
RNA
Small Interfering
Sequence Deletion
Cell Nucleus
Phosphorylated Histone H2AX
Tumor Suppressor Proteins
DNA Helicases
Cell Biology
Histone acetyltransferase
Chromatin
Cell biology
DNA-Binding Proteins
Angiopoietin-like Proteins
medicine.anatomical_structure
chemistry
Fatty Acids
Unsaturated
biology.protein
ATPases Associated with Diverse Cellular Activities
Angiopoietins
Nucleus
DNA
HeLa Cells
Protein Binding
Zdroj: Experimental Cell Research. 315:206-217
ISSN: 0014-4827
DOI: 10.1016/j.yexcr.2008.10.028
Popis: Certain actin-related proteins (Arps) of budding yeast are localized in the nucleus, and have essential roles as stoichiometric components of histone acetyltransferase (HAT) and chromatin remodeling complexes. On the other hand, identification of vertebrate nuclear Arps and their functional analyses are just beginning. We show that human Arp5 (hArp5) proteins are localized in the nucleus, and that arp5Delta yeast cells are partially complemented by hArp5. Thus, hArp5 is a novel member of the nuclear Arps of vertebrates, which possess evolutionarily conserved functions from yeast to humans. We show here that hArp5 shuttles between the nucleus and the cytoplasm. Furthermore, after the induction of DNA double strand breaks (DSB), cell growth and the accumulation of phosphorylated histone H2AX (gamma-H2AX) are impaired by hArp5 depletion. Association of hArp5 with the hIno80 chromatin remodeling enzyme and decrease of chromatin-bound hIno80 by hArp5-depletion indicate that hArp5 may have a role in the recruitment of the hINO80 complex to chromatin. Overexpression of hArp5 and hIno80 enhanced gamma-H2AX accumulation. These observations suggest that hArp5 is involved in the process of DSB repair through the regulation of the chromatin remodelling machinery.
Databáze: OpenAIRE
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