Isolation of intracytoplasmic membrane from the methanotrophic bacterium Methylomicrobium album BG8
| Autor: | Mary Lynne Perille Collins, Christine A. Brantner, Lorie A. Buchholz, Charles C. Remsen |
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| Rok vydání: | 1999 |
| Předmět: |
Methane monooxygenase
Membrane Proteins General Medicine Intracellular Membranes Biology biology.organism_classification Cell Fractionation Applied Microbiology and Biotechnology Microbiology Membrane Biochemistry Cytoplasm Methylococcaceae biology.protein Centrifugation Density Gradient Oxygenases Centrifugation Cell fractionation Cell envelope Bacterial outer membrane Bacteria Copper |
| Zdroj: | Current microbiology. 40(2) |
| ISSN: | 0343-8651 |
| Popis: | Methane-oxidizing bacteria, including Methylomicrobium album BG8, form an intracytoplasmic membrane in addition to the cytoplasmic and outer membranes of the cell envelope. Techniques to isolate the intracytoplasmic membrane of M. album BG8 were developed. An intracytoplasmic membrane fraction was separated from a cell envelope fraction on the basis of sedimentation velocity in sucrose density gradients. Proteins associated with the particulate methane monooxygenase were found in both membrane fractions. |
| Databáze: | OpenAIRE |
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