Immunosenescence profile and expression of the aging biomarker (p16INK4a) in testicular cancer survivors treated with chemotherapy
Autor: | María T. Bourlon, Diego F. Hernández-Ramírez, Guadalupe Lima, Luis Orozco, Y Atisha-Fregoso, Luis Llorente, Hugo E Velázquez, Irene Medina-Rangel, Juan Hinojosa, Ricardo Rios-Corzo, Francisco J. Valentin-Cortez |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Male Cancer Research Aging Lymphocyte Testicular cancer survivors CD8-Positive T-Lymphocytes 0302 clinical medicine Cancer Survivors Medicine biology medicine.diagnostic_test Immunosenescence Middle Aged Neoplasms Germ Cell and Embryonal lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens medicine.anatomical_structure Oncology Premature aging 030220 oncology & carcinogenesis Female Research Article Adult Germ cell tumors Peripheral blood mononuclear cell lcsh:RC254-282 CD19 Flow cytometry Andrology 03 medical and health sciences Molecular aging p16INK4a Testicular Neoplasms Testicular cancer Genetics Humans neoplasms Cyclin-Dependent Kinase Inhibitor p16 business.industry fungi medicine.disease 030104 developmental biology biology.protein Leukocytes Mononuclear business CD8 |
Zdroj: | BMC Cancer BMC Cancer, Vol 20, Iss 1, Pp 1-7 (2020) |
ISSN: | 1471-2407 |
Popis: | Background Cytotoxic chemotherapy can cure advanced germ cell tumors. Nevertheless, cancer treatment may induce cellular senescence and accelerate molecular aging. The aging process implies an increase of cells expressing p16INK4a and changes in lymphocyte subpopulations. Our aim was to study the potential induction of premature immunosenescence in testicular cancer survivors (TCS) exposed to chemotherapy. Methods Case-control exploratory study of TCS treated with chemotherapy (≥3 BEP cycles, disease-free ≥3 months) compared with age matched healthy controls. Peripheral blood mononuclear cells were isolated, and lymphocyte subpopulations were analyzed by flow cytometry. CDKN2A/p16INK4a expression in T cells was measured using qPCR. The percentage of lymphocyte subpopulations and the CDKN2A/p16INK4a expression in TCS were compared with the control group using the Wilcoxon signed-rank test. Results We included 16 cases and 16 controls. The median age was 27 years (minimum 24, maximum 54) and the median time on surveillance was 26.5 months (minimum 3, maximum192). TCS had a lower percentage of total T cells and CD4+ T cells in total lymphocytes. Among the CD4+ T lymphocytes, TCS had less naïve CD4+ and increased memory CD4+ cells. Within the CD8+ T lymphocytes, TCS exhibited a decrease in the percentage of naïve cells and an increase in CD8 + CD45RA + CD57+ cells. TCS also exhibited decreased memory CD19+ B cells compared to the controls. The relative expression of CDKN2A/p16INK4a in T cells was increased in TCS (mean 1.54; 95% CI of the mean: 1.074–2.005; p = 0.048). Conclusion In this exploratory study, TCS showed increased expression of CDKN2A/p16INK4a and a lymphocyte phenotype that has been associated with immunosenescence. Further studies are warranted to define the clinical implications of these alterations in TCS. |
Databáze: | OpenAIRE |
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