Glycosphingolipid-associated β-1,4 galactosyltransferase is elevated in patients with systemic lupus erythematosus
| Autor: | Vignesh Sadras, Michelle Petri, Subroto Chatterjee, Barbara Lee Peterlin, Steven R. Jones |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine Apolipoprotein B chemistry.chemical_compound 0302 clinical medicine systemic lupus erythematosus Risk Factors Lupus Erythematosus Systemic Systemic lupus erythematosus music.instrument biology Biomarker Studies General Medicine Glycosphingolipid Middle Aged Galactosyltransferases Antibodies Antiphospholipid Female medicine.symptom Antibody Adult lcsh:Immunologic diseases. Allergy medicine.medical_specialty Immunology Lactosylceramides Enzyme-Linked Immunosorbent Assay Inflammation lipids 03 medical and health sciences Lactosylceramide Antigen Internal medicine medicine Humans music 030203 arthritis & rheumatology business.industry Atherosclerosis medicine.disease 030104 developmental biology Endocrinology chemistry inflammation Case-Control Studies Baltimore biology.protein business lcsh:RC581-607 Lipoprotein(a) Lipoprotein |
| Zdroj: | Lupus Science and Medicine, Vol 7, Iss 1 (2020) Lupus Science & Medicine |
| ISSN: | 2053-8790 |
| Popis: | Objectiveβ-1,4 galactosyltransferase-V (β-1,4 GalT-V) is an enzyme that synthesises a glycosphingolipid known as lactosylceramide, which has been implicated in general inflammation and atherosclerosis. We asked if β-1,4 GalT-V was present at elevated levels in patients with SLE, a disease which is associated with increased risk of atherosclerosis.MethodsIn this case–control observational study, serum samples were obtained from patients with SLE who are part of the Johns Hopkins Lupus Cohort. Control serum samples were obtained from healthy adult community members recruited from the Baltimore area. All serum samples (n=50 in the SLE group and n=50 in the healthy control group) were analysed with enzyme-linked immunoassays. These assays used antibodies raised against antigens that enabled us to measure the absorbance of oxidised phosphocholines per apolipoprotein B-100 (ox-PC/apoB) and the concentration of lipoprotein(a) (Lp(a)) and β-1,4 GalT-V.ResultsAbsorbance of ox-PC/apoB and concentrations of Lp(a) and β-1,4 GalT-V were significantly higher in the SLE serum samples as compared with the control serum (pConclusionsWe conclude that patients with SLE have elevated levels of β-1,4 GalT-V and ox-PC, which have previously been recognised as risk factors for atherosclerosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|