The Molecular Background of the Differential UV Absorbance of the Human Lens in the 240-400 nm Range
| Autor: | Lajos Kolozsvári, Sung Ung Kang, Akos Tiboldi, Kongzhao Li, Gert Lubec, Béla Hopp, Viktor Pajer, Narkhyun Bae, Antal Nógrádi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Adult
Gel electrophoresis Chromatography medicine.diagnostic_test Ultraviolet Rays Spectrophotometry Atomic General Medicine Middle Aged Biology Mass spectrometry Trypsin medicine.disease_cause Biochemistry Lens protein Electrophoresis medicine.anatomical_structure Spectrophotometry Lens (anatomy) Lens Crystalline medicine Humans Electrophoresis Gel Two-Dimensional Physical and Theoretical Chemistry Ultraviolet medicine.drug |
| Zdroj: | Photochemistry and Photobiology. 89:856-863 |
| ISSN: | 0031-8655 |
| DOI: | 10.1111/php.12063 |
| Popis: | The ultraviolet (UV) absorption of various sections of the human lens was studied and compared with protein expression paralleling differential UV absorbance in anterior and posterior lenticular tissue. The UV absorbance of serial lens cryostat sections (60 μm) and that of lens capsules was determined using a Shimadzu scanning spectrophotometer, and the absorption coefficients were calculated. Two-dimensional gel electrophoresis was performed using two pooled lenticular protein extracts (anterior and posterior sections). Protein spots were quantified and significantly different spots were identified by mass spectrometry following in-gel digestion with trypsin and chymotrypsin. The UV-C and UV-B absorption of the human lens increased toward the posterior parts of the lens. The anterior and posterior lens capsules also effectively absorbed UV radiation. Levels of molecular chaperone proteins Beta-crystallin B2 (UniProtKB ID:P43320), A3 (UniProtKB ID:P05813) and of glyceraldehyde 3-phosphate dehydrogenase (UniProtKB ID:P04406) were significantly higher in the anterior part of the lens, whereas lens proteins Beta-crystallin B1 (UniProtKB ID:P53674) and Alpha-crystallin A chain (UniProtKB ID:P02489) were higher in the posterior sections. These results provide evidence that differential UV absorption in the anterior and posterior lens is accompanied by differential protein expression. |
| Databáze: | OpenAIRE |
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