Translational termination without a stop codon
| Autor: | Nathan R. James, Venki Ramakrishnan, Alan Brown, Yuliya Gordiyenko |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Conformation Translational termination Bioinformatics Ribosome Article 03 medical and health sciences Escherichia coli 3' Untranslated Regions Messenger RNA Multidisciplinary Protein Stability Chemistry Escherichia coli Proteins Cryoelectron Microscopy RNA-Binding Proteins Peptide Chain Termination Translational Ribosomal RNA Stop codon Cell biology RNA Bacterial A-site Open reading frame 030104 developmental biology RNA Ribosomal Codon Terminator Peptides Release factor Ribosomes Peptide Termination Factors |
| Zdroj: | Science. 354:1437-1440 |
| ISSN: | 1095-9203 0036-8075 |
| DOI: | 10.1126/science.aai9127 |
| Popis: | Rescuing stalled ribosomes A small percentage of bacterial mRNAs lack a stop codon. Ribosomes stall at the end of such mRNAs, and the buildup of stalled ribosomes can be lethal. The primary rescue mechanism, in which translation continues on a piece of RNA that contains a stop codon, is a drug target. However, bacteria have another backup plan. James et al. present structures that show that ArfA (alternative rescue factor A) substitutes for a stop codon by binding in the ribosomal mRNA channel and recruiting RF2 (release factor 2). It mediates conformational changes required for RF2 to catalyze peptide release. Science , this issue p. 1437 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|