AMY-1, a c-Myc-binding protein, is localized in the mitochondria of sperm by association with S-AKAP84, an anchor protein of cAMP-dependent protein kinase
| Autor: | Sanae M.M. Iguchi-Ariga, Takahiro Taira, Makoto Furusawa, Hiroyoshi Ariga, Takako Ohnishi |
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| Rok vydání: | 2001 |
| Předmět: |
Male
health care facilities manpower and services Protein subunit Gi alpha subunit A Kinase Anchor Proteins Mitogen-activated protein kinase kinase Biochemistry Cell Line Proto-Oncogene Proteins c-myc Mice Two-Hybrid System Techniques Testis Animals Humans Tissue Distribution cardiovascular diseases c-Raf Protein kinase A Fluorescent Antibody Technique Indirect Spermatogenesis Molecular Biology health care economics and organizations Adaptor Proteins Signal Transducing biology Reverse Transcriptase Polymerase Chain Reaction Cyclin-dependent kinase 2 Membrane Proteins Cell Biology Autophagy-related protein 13 Blotting Northern Molecular biology Cyclic AMP-Dependent Protein Kinases Spermatozoa Mitochondria DNA-Binding Proteins Alternative Splicing Protein Transport Salivary alpha-Amylases Cyclin-dependent kinase complex biology.protein Carrier Proteins HeLa Cells Plasmids Protein Binding Transcription Factors |
| Zdroj: | The Journal of biological chemistry. 276(39) |
| ISSN: | 0021-9258 |
| Popis: | We have reported that a novel c-Myc-binding protein, AMY-1 (associate of Myc-1), stimulated the transcription activity of c-Myc. To access the molecular function of AMY-1, a two-hybrid screening of cDNAs encoding AMY-1-binding proteins was carried out with AMY-1 as a bait using a human HeLa cDNA library, and a clone encoding cAMP-dependent protein kinase anchor protein 149 (AKAP149), was obtained. AMY-1 was found to bind in vitro and in vivo to the regulatory subunit II binding region of AKAP149 and S-AKAP84, a splicing variant of AKAP149 expressed in the testis. AMY-1 was expressed postmeiotically in the testis, as S-AKAP84 was expressed. Furthermore, S-AKAP84 and regulatory subunit II, a regulatory subunit of cAMP-dependent protein kinase, made a ternary complex in cells, and AMY-1 was localized in the mitochondria of HeLa and sperm in association with AKAP149 and S-AKAP84, respectively. These results suggest that AMY-1 plays a role in spermatogenesis. |
| Databáze: | OpenAIRE |
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