Up-regulation of P-glycoprotein expression by osmotic stress in rat sugar cataract
| Autor: | Yoshio Akagi, Yoshihiro Takamura, Eri Kubo, T. Miyazawa |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Osmosis
Cell type genetic structures Osmotic shock Polymerase Chain Reaction Cataract Rats Sprague-Dawley Cellular and Molecular Neuroscience Polyol pathway Downregulation and upregulation Aldehyde Reductase Lens Crystalline Animals ATP Binding Cassette Transporter Subfamily B Member 1 RNA Messenger Eye Proteins Cells Cultured P-glycoprotein Aldose reductase biology Chemistry Galactose eye diseases Sensory Systems Rats Up-Regulation Cell biology Ophthalmology Biochemistry Chloride channel biology.protein Osmoregulation Female sense organs |
| Zdroj: | Experimental Eye Research. 84:246-253 |
| ISSN: | 0014-4835 |
| DOI: | 10.1016/j.exer.2006.09.012 |
| Popis: | P-glycoprotein (P-gp), a plasma membrane protein, is thought to function in the export of cytotoxic drugs and to act as a modulator of chloride channels that regulate cell volume in many cell types. P-gp has been shown to play a role in lens volume regulation and initiation of osmotic cataract. We investigated the lenticular expression levels of P-gp in galactose-fed rats, an experimental model of sugar cataract. P-gp was overexpressed in lenses from galactose-fed rats with cortical sugar cataract, and in rat lens epithelial cells cultured in high-glucose medium. However, application of aldose reductase (AR) inhibitor was able to reverse the changes in P-gp levels in the lenses of galactose-fed rats, confirming the role of AR and involvement of the polyol pathway in cataract formation. Our findings suggest that P-gp may be induced by AR over-expression and/or osmotic stress, thus playing a regulatory role in maintaining lenticular osmotic balance in sugar cataract. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect