In vitro-deranged intestinal immune response to gliadin in type 1 diabetes
| Autor: | Francesco Paparo, S. Percopo, Francesca Lombardi, Luigi Greco, Riccardo Troncone, Gerardo Nardone, Renata Auricchio, Maria Maglio, Giuliana Valerio, Adriana Franzese |
|---|---|
| Přispěvatelé: | Auricchio, Renata, Paparo, F, Maglio, M, Franzese, Adriana, Lombardi, F, Valerio, G, Nardone, GERARDO ANTONIO PIO, Percopo, S, Greco, Luigi, Troncone, R. |
| Rok vydání: | 2004 |
| Předmět: |
Male
Adolescent Endocrinology Diabetes and Metabolism Biopsy Inflammation digestive system Gliadin Immune system Organ Culture Techniques HLA Antigens Immunopathology Internal Medicine medicine Humans IL-2 receptor Intestinal Mucosa Child Autoimmune disease Type 1 diabetes Lamina propria biology business.industry medicine.disease Immunohistochemistry digestive system diseases medicine.anatomical_structure Diabetes Mellitus Type 1 Jejunum Case-Control Studies Immunology Antibody Formation biology.protein Female medicine.symptom business |
| Zdroj: | Diabetes. 53(7) |
| ISSN: | 0012-1797 |
| Popis: | Dietary gluten has been associated with an increased risk of type 1 diabetes. We have evaluated inflammation and the mucosal immune response to gliadin in the jejunum of patients with type 1 diabetes. Small intestinal biopsies from 17 children with type 1 diabetes without serological markers of celiac disease and from 50 age-matched control subjects were examined by immunohistochemistry. In addition, biopsies from 12 type 1 diabetic patients and 8 control subjects were cultured with gliadin or ovalbumin peptic-tryptic digest and examined for epithelial infiltration and lamina propria T-cell activation. The density of intraepithelial CD3(+) and gammadelta(+) cells and of lamina propria CD25(+) mononuclear cells was higher in jejunal biopsies from type 1 diabetic patients versus control subjects. In the patients' biopsies cultured with peptic-tryptic gliadin, there was epithelial infiltration by CD3(+) cells, a significant increase in lamina propria CD25(+) and CD80(+) cells and enhanced expression of lamina propria CD54 and crypt HLA-DR. No such phenomena were observed in control subjects, even those with celiac disease-associated HLA haplotypes. In conclusion, signs of mucosal inflammation were present in jejunal biopsies from type 1 diabetic patients, and organ culture studies indicate a deranged mucosal immune response to gliadin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|