Membrane Bending Modulus and Adhesion Energy of Wild-Type and Mutant Cells of Dictyostelium Lacking Talin or Cortexillins
| Autor: | Erich Sackmann, Rudolf Simson, Günther Gerisch, Jens Niewöhner, Jan Faix, Eva Wallraff |
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| Rok vydání: | 1998 |
| Předmět: |
Talin
biology Flexural modulus Cell Membrane Microfilament Proteins Protozoan Proteins Wild type Biophysics biology.organism_classification Actins Elasticity Dictyostelium discoideum Cell biology Membrane bending Cell membrane Membrane medicine.anatomical_structure Cell Adhesion medicine Animals Dictyostelium Cell adhesion Mathematics Actin Research Article |
| Zdroj: | Biophysical Journal. 74(1):514-522 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/s0006-3495(98)77808-7 |
| Popis: | We have employed an interferometric technique for the local measurement of bending modulus, membrane tension, and adhesion energy of motile cells adhering to a substrate. Wild-type and mutant cells of Dictyostelium discoideum were incubated in a flow chamber. The flow-induced deformation of a cell near its adhesion area was determined by quantitative reflection interference contrast microscopy (RICM) and analyzed in terms of the elastic boundary conditions: equilibrium of tensions and bending moments at the contact line. This technique was employed to quantify changes caused by the lack of talin, a protein that couples the actin network to the plasma membrane, or by the lack of cortexillin I or II, two isoforms of the actin-bundling protein cortexillin. Cells lacking either cortexillin I or II exhibited reduced bending moduli of 95 and 160 k(B)T, respectively, as compared to 390 k(B)T, obtained for wild-type cells. No significant difference was found for the adhesion energies of wild-type and cortexillin mutant cells. In cells lacking talin, not only a strongly reduced bending modulus of 70 k(B)T, but also a low adhesion energy one-fourth of that in wild-type cells was measured. |
| Databáze: | OpenAIRE |
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