Gui-A-Gra Attenuates Testicular Dysfunction in Varicocele-Induced Rats Via Oxidative Stress, ER Stress and Mitochondrial Apoptosis Pathway
| Autor: | Hye Kyung Kim, Chul Kim, Keshab Kumar Karna, Yu Seob Shin, Jong Kwan Park, Na Young Choi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine steroidogenic acute regulatory (StAR) protein Varicocele endoplasmic reticulum (ER) stress medicine.disease_cause lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine oxidative stress lcsh:QH301-705.5 Spectroscopy chemistry.chemical_classification TUNEL assay biology Glutathione peroxidase Mitophagy apoptosis General Medicine Endoplasmic Reticulum Stress Malondialdehyde Immunohistochemistry Spermatozoa Computer Science Applications mitochondria 030220 oncology & carcinogenesis Gui-A-Gra Sperm Motility Inflammation Mediators Oxidoreductases varicocele medicine.medical_specialty testis Article Catalysis Inorganic Chemistry Superoxide dismutase 03 medical and health sciences Bcl-2-associated X protein Internal medicine parasitic diseases Edible Insects medicine Animals Physical and Theoretical Chemistry Molecular Biology Reactive oxygen species Organic Chemistry medicine.disease Rats 030104 developmental biology Endocrinology chemistry lcsh:Biology (General) lcsh:QD1-999 inflammation biology.protein Lipid Peroxidation Reactive Oxygen Species Biomarkers Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences, Vol 21, Iss 9231, p 9231 (2020) International Journal of Molecular Sciences; Volume 21; Issue 23; Pages: 9231 International Journal of Molecular Sciences |
| ISSN: | 1661-6596 1422-0067 |
| Popis: | Gui-A-Gra, a commercial insect powder from Gryllus bimaculatus, is registered as an edible insect by the Korean food and drug administration. The aim of this study was to investigate the effect of Gui-A-Gra on testicular damage induced by experimental left varicocele in male Sprague Dawley rats. A total of 72 rats were randomly divided into the following six groups (12 rats in each group): a normal control group (CTR), a group administrated with Gui-A-Gra 1.63 gm/kg (G1.63), a group administrated with Gui-A-Gra 6.5 gm/kg (G6.5), a varicocele (VC)-induced control group (VC), a VC-induced group administrated with Gui-A-Gra 1.63 gm/kg (VC + G1.63), and a VC-induced group administrated with Gui-A-Gra 6.5 gm/kg (VC + G6.5). Rats were administrated 1.63 or 6.5 gm/kg Gui-A-Gra once daily for 42 days. Indicators of sperm parameters, histopathology, reproductive hormones, oxidative stress, endoplasmic reticulum (ER) stress, inflammation, and mitochondrial apoptosis were analyzed to evaluate effects of Gui-A-Gra on VC-induced testicular dysfunction. Gui-A-Gra administration to VC-induced rats significantly (p < 0.05) increased sperm count and sperm motility, Johnsen score, spermatogenic cell density, serum testosterone, testicular superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase, GPx4, and steroidogenic acute regulatory protein (StAR) level. Moreover, pretreatment with Gui-A-Gra significantly (p < 0.05) decreased terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) positive cells/tubules, serum luteinizing hormone (LH), serum follicle-stimulating hormone (FSH), testicular tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), malondialdehyde (MDA), reactive oxygen species (ROS)/reactive nitrogen species (RNS) level, glucose-regulated protein-78 (Grp-78), phosphorylated c-Jun-N-terminal kinase (p-JNK), phosphorylated inositol-requiring transmembrane kinase/endoribonuclease 1α (p-IRE1α), cleaved caspase-3, and BCL2 associated X protein: B-cell lymphoma 2 (Bax: Bcl2) ratio in VC rats. These results suggest that protective effects of Gui-A-Gra on VC-induced testicular injury might be due to its antioxidant, anti-inflammatory, and androgenic activities that might be mediated via crosstalk of oxidative stress, ER stress, and mitochondrial apoptosis pathway. |
| Databáze: | OpenAIRE |
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