Xenobiotics Produce Distinct Metabolomic Responses in Zebrafish Larvae (Danio rerio)
| Autor: | Heather Butler, Bharat Chandramouli, John R. Cosgrove, Jonathan P. Benskin, Susie S.Y. Huang, Caren C. Helbing |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Embryo Nonmammalian Danio 010501 environmental sciences 01 natural sciences Xenobiotics 03 medical and health sciences chemistry.chemical_compound Metabolomics Zebrafish larvae Animals Environmental Chemistry Zebrafish 0105 earth and related environmental sciences chemistry.chemical_classification Developmental stage biology Ecology Embryo General Chemistry biology.organism_classification Amino acid 030104 developmental biology Biochemistry chemistry Larva Xenobiotic Water Pollutants Chemical |
| Zdroj: | Environmental Science & Technology. 50:6526-6535 |
| ISSN: | 1520-5851 0013-936X |
| DOI: | 10.1021/acs.est.6b01128 |
| Popis: | Sensitive and quantitative protocols for characterizing low-dose effects are needed to meet the demands of 21st century chemical hazard assessment. To test the hypothesis that xenobiotic exposure at environmentally relevant concentrations produces specific biochemical fingerprints in organisms, metabolomic perturbations in zebrafish (Danio rerio) embryo/larvae were measured following 24 h exposures to 13 individual chemicals covering a wide range of contaminant classes. Measured metabolites (208 in total) included amino acids, biogenic amines, fatty acids, bile acids, sugars, and lipids. The 96-120 h post-fertilization developmental stage was the most appropriate model for detecting xenobiotic-induced metabolomic perturbations. Metabolomic fingerprints were largely chemical- and dose-specific and were reproducible in multiple exposures over a 16-month period. Furthermore, chemical-specific responses were detected in the presence of an effluent matrix; importantly, in the absence of morphological response. In addition to improving sensitivity for detecting biological responses to low-level xenobiotic exposures, these data can aid the classification of novel contaminants based on the similarity of metabolomic responses to well-characterized "model" compounds. This approach is clearly of use for rapid, sensitive, and specific analyses of chemical effect on organisms, and can supplement existing methods, such as the Zebrafish Embryo Toxicity assay (OECD TG236), with molecular-level information. |
| Databáze: | OpenAIRE |
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